User:Birdy0124/sandbox

First Practice Post
I never knew of the Italian poet Torquato Tasso (March 11, 1544 – April 25, 1595) until recently.

I was taken on a marvelous tour of the various parts of Italy through a show on PBS. On an old wall read one of Tasso's famous words, perduto e tutto il tempo che in amor non si spende, which translates to lost is all the time that is not engaged in love.

Both wonderful and true.

More Practice: 5 Pillars of Wikipedia

 * 1) Encyclopedia - Wikipedia is to be informative and as objective as possible.
 * 2) Neutral Point of View - To echo the previous point, Wikipedia is to be factual and keep bias to a minimum.
 * 3) Free Content - Anyone can edit, use, modify, distribute. Content not owned by one individual or a specific group.
 * 4) Respect and Civility - Editors should have respect for other contributors and maintain good net-etiquette.
 * 5) No Firm Rules - Adaptive policies and guidelines to maintain Wikipedia objectives.

Summary of Characteristics of Target Article
Good Article [GA] standard indicates that it passed an official review and useful to nearly all readers with no obvious problems, but lacks the quality of professional encyclopedia (e.g., featured article [FA] standard). This means that the article was written well, accurate, verifiable, broad in coverage, neutral in point of view, stable and illustrated. But it lacks Standard has six criteria: well-written, verifiable with no original research, broad in its coverage, neutral, stable, and illustrated if possibly by images.

B Quality standard is mostly complete without major problems. But may need expanded and/or expert information in sections and could use stylistic improvements.

Proteomics and Early Detection (PubMed Assignment)
The prevalence of breast cancer is several times greater than that of ovarian cancer. However, the mortality rate is approximately 19% for breast cancer, compared to 56% in ovarian cancer. Advanced stage diagnosis is a major contributing factor of the high mortality rate. Clinically accepted methods for diagnosis, Serum CA-125 level evaluation and ultrasonography, are not adequate for early detection due to false positive and false negative results. Liang et al. attempts to resolve the proteomic profile of ovarian cancer-derived exosomes, specifically exosomes isolated from cell lines OVCAR-3 and IGROV1. The experiment contained in the paper identified some proteins previously unidentified in exosomes. The experimental procedures highlight how advances in proteomic technology is aiding such research and the findings emphasize the importance of proteomic analysis of exosomes and their role in ovarian cancer. According to the paper, ovarian cancer cell-derived exosomes can: (1) carry cell/tissue-specific proteins that can lead to new new biomarkers for diagnosis, and (2) indicate exosomal functions in ovarian cancer. Of course, diagnostic markers need to be tested, validated and confirmed. But this demonstrates that novel therapeutic approaches can be derived through proteomic analysis. A diagnostic tool sensitive enough to detect and discern the disease early would be a huge step in the right direction for Ovarian Cancer.

Eukaryotic Transcription Outline
I. Making RNA replica of gene

II. Eukaryotic RNA polymerases
 * A. RNA polymerase I
 * B. RNA polymerase II
 * C. RNA polymerase III

III. Initiation
 * A. Eukaryotic promoters
 * B. Regulatory sequences beyond the core promoters
 * C. General transcription factors
 * D. Preinitiation complex
 * E. Promoter melting and close-to-open transition
 * F. Abortive initiation
 * G. Promoter escape

IV. Elongation
 * A. Elongation factors
 * B. Proofreading and transcription fidelity
 * C. RNA processing

V. Termination
 * A. Cleavage and 3'-end polyadenylation
 * B. Second RNA (Torpedo model)

VI. Transcription through nucleosomes

VII. Eukaryotic transcriptional control
 * A. Initiation (Mediator complex, etc)
 * B. Elongation
 * C. Termination

VIII. Comparisons between prokaryotic and eukaryotic transcription

IX. Comparisons to eukaryotic DNA replication

X. Transcription-coupled DNA repair

XI. Eukaryotic transcription protein list

List of High-Quality References

 * II. RNA polymerases Chapter 6 of this book details how transcription occurs by polymerase II, as well as elucidating the roles of polymerase I and III in transcription
 * IV. Eukaryotic protein elongation factors : Access to this journal is restricted but Hopkins students are able to access it. It gives detailed information about Elongation factors 1 and 2 and introduces a a third elongation factor seen in yeast.
 * VI: Mechanism of Transcription through nucleosome : This journal goes into detail by explaining RNA polymerase II single round transcription, overcoming the nucleosome barrier, histone survival and displacement as well transcription through chromatin in vivo.
 * VII: This book gives a more "all round" view of transcriptional regulation by explaining three levels of control: activators and repressors activities; changes in chromatin structure ; and direct influence of activators and repressors on initiator complexes
 * X: Mechanism of Transcription-Coupled DNA Repair: This reference may go too much in detail but we should be able to glean the basics of TCR repair. It is also said to provide the knowledge of TCR from the point of view of eukaryotic transcription as well as providing current models of for how the TCR system works.
 * The transcription cycle in eukaryotes: From productive initiation to RNA polymerase II recycling. Most other papers portrait transcription as separate, disjointed steps, this paper views the eukaryotic transcription as a dynamic cycle. This will be a thread we use to unit the different sections of the article.)
 * Disentangling the many layers of eukaryotic transcriptional regulation. Recent review on the eukaryotic transcriptional regulation, focusing on the cis-regulatory modules (DNA/genetic) and nucleosomes (histone epigenetic). This may not be included as a reference in the final article, but we will need something like this.
 * General source of Eukaryotic Transcription information . It is not as general as one may think. Contains a wealth of information on the details of transcription mechanisms, and is a source of many diagrams
 * This is the background text for most of the topics headings that have been outlined