User:Blobling/sandbox

Rate Zonal centrifugation is the process in which particles sediment though a medium of lower density but stop when they reach a higher density. The reason we use rate zonal as a technique is so the particles that are used do not cross contaminate so the fast sedimenting particles are not contaminated by the slow sedimenting particles. The fall back of this technique is that the narrow load limits the volume of sample (typically 10%) and if you use centrifuge for an extended period of time you will no longer have the separation of densities as all will be pelleted together.