User:Brianferrera9/sandbox

The variant trichrome is applied by submerging the fixated sample into the following three Solutions:

1) Weigert's Iron Hematoxylin working solution

2) Biebrich Scarlet solution

3) Fast Green FCF solution

(Note: Define how above solutions are made)

The resulting stains are black cell nuclei, brown cytoplasm, red muscle and myelinated fibers, blue collagen, and scarlet erythrocytes.

Standard Applications: Trichrome stains are normally used to differentiate between collagen and muscle tissues. Some studies that benefit from its application include end stage liver disease (cirrhosis), myocardial infarction, muscular dystrophy, and tumor analysis.

(Note: Future insert for Lillie's trichrome )