User:Celina Sewlochan/sandbox

Original- "Quorum sensing"

Quorum quenching
Quorum quenching is the process of preventing quorum sensing by disrupting the signaling. This may be achieved by degrading the signalling molecule. Using a KG medium, quorum quenching bacteria can be readily isolated from various environments including that which has previously been considered as unculturable. Recently, a well-studied quorum quenching bacterium has been isolated and its AHL degradation kinetic has been studied by using rapid resolution liquid chromatography (RRLC).

Quorum quenching
Quorum quenching is the process of preventing quorum sensing by disrupting the signaling. This may be achieved by inactivating enzymes involved in signaling, by introducing molecules that mimic the signaling molecules and block the receptors, or by degrading the signaling molecules themselves. Closantel and triclosan are known inhibitors of quorum sensing enzymes .The former interferes with the histamine kinase sensor in two-component signalling, and the latter disrupts the synthesis of AHL by blocking the enoyl-acyl carrier protein (ACP) reductase. Two groups of well-known mimicking molecules include halogenated furanones, which mimic AHL molecules, and synthetic Al peptides (AIPs), which mimic naturally occurring AIPs. These groups inhibit receptors from binding substrate or decrease the concentration of receptors on the cell. Recently, a well-studied quorum quenching bacterial strain (KM1S) was isolated and its AHL degradation kinetic was studied using rapid resolution liquid chromatography (RRLC). RRLC efficiently separates components of a mixture based on their affinity for different liquid phases to a high degree of sensitivity. It was found that the genome of this strain encoded an inactivation enzyme with distinct motifs targeting the degradation of N-acyl homoserine lactones (a class of signaling molecules). Applications of quorum quenching that have been exploited by humans include the use of AHL-degrading bacteria in aquacultures to limit the spread of diseases in aquatic populations of fish, mollusks and crustaceans. This technique has also been translated to agriculture to restrict the spread of bacterial diseases in plants. Anti-biofouling is another process that mediates the dissociation of unwanted biofilms aggregating on wet surfaces, such as medical devices, transportation infrastructure and water systems. It has been found that some microorganisms, such as Flustra foliacea, that inhabit the same environment as biofouling bacteria, can express quorum quenching traits. F. foliacea secrete 10 brominated alkaloids, two of which contribute to quorum sensing inhibition. Celina Sewlochan (talk) 23:18, 8 October 2017 (UTC)

Quorum quenching
Quorum quenching is the process of preventing quorum sensing by disrupting signalling. This is achieved by inactivating singalling enzymes, by introducing molecules that mimic signalling molecules and block their receptors, or by degrading signalling molecules themselves.

Closantel and triclosan are known inhibitors of quorum sensing enzymes .The former induces aggregation of the histamine kinase sensor in two-component signalling. The latter disrupts the synthesis of a class of signalling molecules known as N-acyl homoserine lactones (AHLs) by blocking the enoyl-acyl carrier protein (ACP) reductase.

Two groups of well-known mimicking molecules include halogenated furanones, which mimic AHL molecules, and synthetic Al peptides (AIPs), which mimic naturally occurring AIPs. These groups inhibit receptors from binding substrate or decrease the concentration of receptors in the cell. Furanones have also been found to act on AHL-dependant transcriptional activity, whereby the half life of the autoinducer-binding LuxR protein is significantly shortened.

Recently, a well-studied quorum quenching bacterial strain (KM1S) was isolated and its AHL degradation kinetic was studied using rapid resolution liquid chromatography (RRLC). RRLC efficiently separates components of a mixture to a high degree of sensitivity, based on their affinities for different liquid phases. It was found that the genome of this strain encoded an inactivation enzyme with distinct motifs targeting the degradation of AHLs.

Applications of quorum quenching that have been exploited by humans include the use of AHL-degrading bacteria in aquacultures to limit the spread of diseases in aquatic populations of fish, mollusks and crustaceans. This technique has also been translated to agriculture, to restrict the spread of pathogenic bacteria that use quorum sensing in plants. Anti-biofouling is another process that exploits quorum quenching bacteria to mediate the dissociation of unwanted biofilms aggregating on wet surfaces, such as medical devices, transportation infrastructure and water systems. Celina Sewlochan (talk) 06:15, 17 November 2017 (UTC)