User:Chemistry phd/sandbox

3-Bromomaleimides and 3,4-dibromomaleimides have been used successfully to bioconjugate a variety of functional molecules to both single cysteines and disulfide bonds in proteins and peptides. These molecules include proteins, peptides, sugars, fluorophores, and polyethylene glycol. The conjugation can be made reversible or irreversible to an excess of thiol by hydrolysis. In the case of single cysteine conjugation, reversible conjugation has been demonstrated in the cytoplasm of mammalian cells.