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Line Cross Analysis

Line cross analyses are an approach to uncover the genetic architecture of a quantitative phenotypic trait like height. This approach was first developed agricultural breeders attempting to maximize the quality of domestic animal production and farming. Line cross analysis is accomplished by breeding two parents with very different values for the trait of interest and producing and F1 generation. This first generation is then used to breed back to the parents and to itself. This creates many groups of individuals all with similar genotypes each of these groups is a line.