User:Cseibert832/sandbox

Single-cell chromatin conformation capture[edit]
Chromosome conformation capture techniques (often abbreviated to 3C technologies or 3C-based methods) are a set of molecular biology methods used to analyze the spatial organization of chromatin in a cell. These methods quantify the number of interactions between genomic loci that are nearby in three dimensional space, but may be separated by many nucleotides in the linear genome.

All 3C methods start with a similar set of steps, performed on a sample of cells. First, the cell genomes are cross-linked, which introduces bonds that "freeze" interactions between genomic loci. The genome is then cut into fragments. Next, random ligation is performed. Lastly, the fragments  are sequenced to determine their proximity to each other (fragments are more likely to be ligated to nearby fragments.)

In single-cell 3C, this last step has typically been done using high-throughput sequencing (Hi-C). Although the recovery rate is as low as 2.5% of potential interactions, it has been possible to generate three dimensional maps of entire genomes using this method.