User:Dniprostar/sandbox

Definition:
Uncultivated (also unculturable?) microorganisms are not those that could not be cultured, rather there is an absence of knowledge about their biology.

Why uncultivated:
Microbial Ecology:

Research:
The cultivation has been improved by using improvements in media such as agar. There has been a significant improvement in agar, by the discovery of an autocalving mistake. (REF 1) (That has improved cultivability in following ways, in those quantitative parameters.)

First approach is to study the biological macromolecules that are sampled from the the environment that these organism thrive in. These include Nucleic Acids (DNA, RNA) and metabolites. These approaches includes microbiomics, single cell sequencing and etc.

Second approach is imitating environmental conditions of these microorganisms. This second approach also branches into putting microorganisms back into their own environment after initial incubation and co-culturing it with other bacteria so that ecological environment is preserved. Bacteria often thrive only when in symbiotic relationship with other bacteria. This keeps the bacteria that is normally around the microorganism being studied.

Microoganisms invariably leave behind their waste and metabolites into their habitat whether it is water, soil or human gut. These include DNA, RNA, metabolites and etc. By analyzing DNA that is floating around in seawater, soil or human gut, we can get a good idea about the microorganism living in the environment.

It is possible to obtain a lot of information about the metabolism, genetics using the environmental sampling methods. However such approach is incomplete. Biochemical pathways and gene expression of these microorganisms require culvation.

Both two approaches are fundamentally different and necessary for to the understand the whole picture.

Application:
NovoBiotic Pharmacuticals: