User:Doctor ZR/Sandbox

Gli Similar 3
Gli similar 3 (Glis3) is a Krüppel-like zinc finger protein implicated in pancreatic beta-cell development, osteoblast differentiation, renal disease, and neonatal diabetes and congenital hypothyroidism (NDH).

Glis3 Structure
Mouse Glis3 encodes a 935 amino acid protein containing five tandem C2H2-type zinc finger motifs which share high levels of homology with the zinc finger domains of other Krüppel-like zinc finger proteins, Gli, Zic, and gfl/lmd. The five zinc fingers of Glis3, located between amino acids 501 and 650, match the C2H2 consensus, C-X4-C-X12,15-H-X3,4-H, with the Krüppel-like interfinger sequence, (T/S)GEKP(Y/F)X. While Glis3 exhibits little homology outside the zinc finger region, a transcriptional activation domain is located between amino acids 756 and 935 at the Glis3 C-terminus.

Glis3 Expression
Northern blot analyses revealed that Glis3 was expressed primarily in the kidney, pancreas, and uterus while in situ hybridization detected Glis3 as early as E8.0.