User:Dorian Pietraru/Allysine

Large quantities of elastin and collagen present in tissue may lead to metastasis: spread of disease. Fibrous tissue containing oxidized collagen may result in a condition known as fibrosis. The oxidation of lysine resides present in collagen creates the aldehyde, aminoadipic-δ-semialdehyde (allysine). Increased allysine concentration in tissues has been correlated to the presence of fibrosis.

In one study, allysine is first reacted in acidic conditions (6M HCl, 110 °C, 24 h) with sodium 2-naphthol-7-sulfonate. A fluorescent bis-naphtol allysine is the product. Allysine is then quantified through use of high-performance liquid chromatography (HPLC). The results of this study provide a statistically relevant method in correlating greater concentrations of allysine and fibrotic tissue. The study shows that fibrotic tissue contains 2.5 times more alyssine than non-fibrotic tissue.