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The species Bordetella trematum was identified in 1996 by comparison of 10 strains of B. trematum against other well characterized Bordetella and Alcaligenes species. "Trema" refers to something pierced or penetrated, or to a gap. "Trematum" pertains to open things, and refers to the presence of bacteria in wounds and other exposed parts of the body. Strain LMG 13506T is the reference strain for this species.

Isolation
Bordetella species typically infect the respiratory tract of humans, but B. trematum has never been isolated from a human or animal respiratory tract. It has been isolated from human ear infection and limb wounds. Below is a list of known B. trematum strains, alternative strain designation, and the date and source where they were first isolated :

Characteristics
B. trematum is a gram negative, capsulated, non-spore forming, rod that is 0.5 μm wide and 1-1.8 μm long, but rods as long as 2.4 μm have been observed. It grows aerobically at temperatures ranging from 25-42°C, with optimal temperature for growth ranging from 35-37°C. Microaerobic growth may be observed at optimal temperatures. It is motile via peritrichous flagella. B. trematum is catalase positive. It is unique in being oxidase negative, since all other species of Bordetella are oxidase positive.

B. trematum may be cultured on horse blood agar, MacConkey agar, and Bordet-Gengou agar. When grown on Bordet-Gengou agar, which is the standard agar for Bordetella isolation, colonies are convex, pearly, smooth, almost transparent, glistening, and are surrounded by a zone of hemolysis. When grown on blood agar, colonies are grayish cream white, circular, and convex.

Metabolism
Little is known about the metabolism of the species B. trematum since it is relatively new. Like all Bordetella species, B. trematum is a chemoorganotroph and requires nicotinamide, organic sulfur such as cysteine, and organic nitrogen such as amino acids for growth via respiratory metabolism. It's metabolism is non-saccharolytic, meaning it does not use any form sugar for energy. Six out of the ten known strains are able to reduce nitrate to nitrite. It is indole negative and urease negative.

Taxonomy
DNA-rRNA hybridization was used to place B. trematum LMG 13506T in the Alcaligenaceae family. Amplified Ribosomal DNA Restriction Analysis, revealed a 94% similarity between the B. trematum reference strain and the Bordatella reference species, B. pertussis. Later, additional evidence to determine placement at the genus level came from DNA-DNA hybridization of B. trematum strains LMG 13506 and LMG 14446 against reference strains of other Bordetella and Alcaligenes species. Results showed the species B. trematum to be most like members of the Bordetella genus. SDS-PAGE analysis of whole-cell proteins allowed for development of a dendrogram displaying that Bordetella is not a monophyletic genus. DNA-rRNA hybridization and 16S rRNA sequence analysis shows a close relationship between Bordetella and Alcaligenes. It is often difficult to differentiate between the two genera, in fact some species across the the genera are phenotypically identical.

B. trematum and B. holmesii are the only Bordetella species that do not colonize the respiratory tract. B. pertussis and B. parapertussis are respiratory pathogens that cause Pertussis. B. bronchiseptica and B. avium are respiratory pathogens of other animals. Bordetella hinzii is nonpathogenic and colonizes the respiratory tract of birds. Bordetella holmesii has been isolated from human blood. Members of the Alcaligenes genus colonize humans, but not the respiratory tract, and soil and water.

Genomics
The B. trematum genome was sequenced in 2013, and is currently undergoing annotation. The genome is estimated to possess 4,145 coding sequences with the majority of sequences coding for amino acids,  amino acid derivatives, and carbohydrate pathways. 184 genes are predicted to be involved in membrane transport. It has a 64-65% GC content. The total genome size is not currently known.

Pathogenicity
B. trematum is a nonpathogenic, opportunistic organism whose sole source of isolation is open, human wounds. . It can be ridden from the body without medical intervention.

Bordetella endotoxins are unique to the genus, species, and strain. B. trematum is the only Bordetella species with a semi-rough LPS which contains a single O-unit. The lipid A unit of B. trematum is identical to that of the opportunistic bacteria Bordetella hinzii, but unlike any other Bordetella species. .