User:Dyeh18/sandbox/Activsignal

ActivSignal is an American company that transforms technology into systems biology proteomics tools, located in Natick, Massachusetts. The marquee product, the IPAD platform, can determine the activity of multiple signaling pathways in one reaction.

(Brief Company Background. Small pieces of information such as "key people" and "products" can be added to the column on the right)

History
(More extensive company background)

IPAD Platform
The IPAD platform (Immuno-Paired-Antibody Detection platform) was developed to help academic, pharma and biotech researchers understand the Pathway activation/inhibition profile of cells. This platform assesses the signaling pathway map profile of more than 20 major pathways at once for each sample. The IPAD platform panel includes 70 different human protein targets, measuring protein modifications and expression for each, with coverage of multiple targets per pathway.

The technology uses antibodies against established and well known targets that reflect activities of canonical pathways. The IPAD platform can be viewed as a sophisticated highly multiplexed sandwich ELISA, using proven pairs of antibodies against known phosphorylation sites, e.g. Akt Thr308; cleaved proteins, e.g. cleaved Caspase-3; protein expression, e.g. p21Cip.

The ActivSignal platform is versatile and can accommodate any number of samples from 2 to 96 and above. The assay is performed in a 96-well plate format, thus it is well designed for screening purposes. The IPAD assay can be used to profile samples made from cell culture, frozen tissues, FFPE sections, biopsies including fine needle biopsy, blood, etc. The IPAD assay requires only 150 nanograms, which can be easily taken from samples where available amounts may be limited, e.g. upon laser dissection of tissue.

IPAD Platform Targets

 * BiP
 * c-Myc
 * Calnexin
 * CD44
 * Chop
 * Cleaved Caspase-3
 * Cleaved Notch
 * Cleaved Parp
 * E-Cadherin
 * Gli1
 * Her2
 * Hsp40
 * Hsp70
 * IκBα
 * p21 Waf1/Cip1
 * p27 Kip1
 * PDI
 * phospho-4EBP1 Thr37/46
 * phospho-Akt1 Ser473
 * phospho-Akt1 Thr308
 * phospho-AMPK Thr172
 * phospho-cAbl Tyr245
 * phospho-cCBL Tyr700
 * phospho-Cdc2 Tyr15
 * phospho-Chk2 Thr68
 * phospho-cKit Tyr730
 * phospho-Cofilin Ser3
 * phospho-Cyclin D1 Thr286
 * phospho-EGFR Tyr1068
 * phospho-FAK Tyr397
 * phospho-GSK3β Ser9
 * phospho-H2AX Ser139
 * phospho-Her3/ErbB3 Tyr1289
 * phospho-Hsp27 Ser78/82
 * phospho-IGF1R Tyr1135/1136
 * phospho-IKKα/β Ser176/180
 * phospho-IRAK4 Thr345/Ser346
 * phospho-IRS1 Ser1101
 * phospho-Jak2 Tyr1008
 * phospho-JNK Thr183/Tyr185
 * phospho-Lck Tyr505
 * phospho-MEK1 Ser217/221
 * phospho-MET Tyr1234/1235
 * phospho-mTOR Ser2448
 * phospho-NF-κB p65 Ser536
 * phospho-Numb Ser276
 * phospho-p38 MAPK Thr180/Tyr182
 * phospho-p44-42 MAPK Thr202/Tyr204
 * phospho-p53 Ser15
 * phospho-p90RSK Ser380
 * phospho-Pak1 Thr423
 * phospho-PDGFRβ Tyr751
 * phospho-PLCγ1 Tyr783
 * Phospho-Rb Ser807/811
 * phospho-Smad1 Ser463/465
 * phospho-Smad2 Ser465/467
 * phospho-Src Tyr416
 * phospho-Stat3 Tyr705
 * phospho-Stat5 Tyr694
 * phospho-Syk Tyr525/526
 * phospho-VEGFR2 Tyr1175
 * phospho-Yap1 Ser127
 * phospho-Zap70 Tyr493
 * phospho-βCatenin Ser33/37/Thr41
 * Socs3
 * SQSTM1/p62
 * TCF1

Pathways Covered
The following is a list of pathways analyzed by ActivSignal


 * Apoptosis signaling
 * Autophagy signaling
 * Cell cycle regulation
 * c-Kit signaling
 * DNA damage response
 * EGFR signaling
 * EMT signaling
 * ERBB2-3 signaling
 * Erk-Mapk signaling
 * Fak-Src Signaling
 * Heat Shock Response
 * HIPPO signaling
 * IGF1R signaling
 * Insulin receptor signaling
 * Jak/Stat signaling
 * mTOR signaling
 * NFkB signaling
 * Notch signaling
 * p38 MAPK signaling
 * PDGFR
 * Pi3k-Akt signaling
 * Sonic Hedgehog signaling
 * TGFβ signaling
 * Unfolded protein response
 * VEGFR signaling
 * Wnt-βCat signaling