User:Eclaverol

E2 electrophysiology technique
Electrophysiology-Enabling (E2) microstructures are recently developed polymeric overlays that integrate micropipette-like microchannels with neuron culture substrates. This technique makes it possible to obtain measurements of electrical activity from developing neurons in vitro in an automated way avoiding the need for micromanipulators and micropipettes.

The E2dish culture-on-a-chip technology represents an evolution with respect to conventional electrophysiology. E2dish microstructured substrates support automated measurement of electrical activity from developing neurons in vitro. Unlike conventional electrophysiology, E2dish does not use micropiettes and micropositioners to approach neurons to record from. Instead, microengineered cell-culture substrates contain microfabricated microchannels acting as embedded micropipettes in which neurons sprout spontaneously, without human intervention, to establish electrophysiological recording configurations.

The concept was first described in 2008 by E.Claverol's neuroengineering team at the Catalonia Institute for Bioengineering

Conventional electrophysiology

Measuring electrical activity produced by Neuron in vitro

Substrate-integrated electrophysiology