User:Eliaspty/practica2

Binding
The initiation of the transcription if a multistep sequential process that involves several mechanisms: promoter location, initial reversible binding of RNA polymerase, conformational changes in RNA polymerase, conformational changes in DNA, binding of nucleoside triphosphate (NTP) to the functional RNA polymerase-promoter complex,  and nonproductive and productive initiation of RNA synthesis.

The promoter binding process is crucial in the understanding of the process of gene expression.

Promoter Location
Although RNA polymerase holoenzyme shows high affinity to non-specific sites of the DNA, this characteristic does not allow us to clarify the process of promoter location. This process of promoter location has been attributed to the structure of the holoenzyme to DNA and  sigma 4 to DNA complexes.