User:Esraaazzam11/Allysine

To qualify protein carbonyls in biological systems, most research studies use the dinitrophenylhydrazine (DNPH) process. The study of particular protein carbonyls, such as allysine, reveals more about specific redox processes and mechanisms at the molecular level. Allysine and its oxidation product, α- amino adipic acid (α-AA), have been discovered to also be diabetes risk indicators. To get a better understanding of this concept, human serum albumin was incubated for 32 hours at 37 degrees Celsius in the presence of FeCl3 (25 μM) and increasing glucose concentrations. Both allysine and α-AA levels were found to rise as glucose levels increased which lead to the depletion of tryptophan. Regarding the underlying functions of tryptophan and its metabolites, depletion of tryptophan and its metabolites may result in additional physiological problems such as coronary artery disease and neurological complications.