User:Ford53/sandbox

Outline for NSF Proposal:
Study of Chimera Hyphae in Mycelium
 * 1) Background Information/Purpose
 * 2) Description of mycelium
 * 3) Structure of Hyphae
 * 4) Mutualism between fungi in plants
 * 5) Explanation how it can help or take away from plants also outlinign the importance of the relationship
 * 6) Discussion of the existence of multiple free floating nuclei of different species forming chimera
 * 7) Dynein-driven molecular movement, a look into the mechanics of the process
 * 8) Multiple organisms being present in the same hyphae provides competitive advantage by providing genetic variation.
 * 9) Competition and mutualism that exist within the hyphae
 * 10) Genetic exchange exist between nuclei within the hyphae
 * 11) Nuclei exchange and movement within the hyphae
 * 12) Competition between nuclei within the the hyphae
 * 13) How are species preventing other species from entering hyphae?
 * 14) Methods for detecting competition
 * 15) Usage of mass spectrometry on soil surrounding hyphae
 * 16) look for environmental toxins preventing competition
 * 17) Sequencing of RNA within the hyphae to determine active gene expression/protein synthesis
 * 18) Look into function of genes in similar fungi
 * 19) Importance in finding if there is a immunological system preventing competing fungi
 * 20) Whole genome sequencing of hyphae to determine diversity
 * 21) phylogenetic comparison
 * 22) Looking into numerous mycelium to detect similarities between species present
 * 23) Isolation of fungi in surrounding soil and sequencing to find all fungi present in area of study to find what specifically is being prevented from entering or allowed to enter hyphae