User:Griffin56a/sandbox

DRAQ5TM is a member of the anthraquinone family of DNA intercalating dyes. DRAQ5 binds in (dA-dT)2 domains with high affinity. DRAQ5 is cell membrane permeant, permitting it to be used on intact cells and tissues as well as those preserved by chemical fixation. DRAQ5 fluoresces in the far-red / near infra-red. DRAQ5 is commonly used in fluorescence microscopy and cell-based assays (for drug discovery) as a nuclear counterstain and in flow cytometry to “gate” nucleated cells from enucleated cells and debris and to permit quantification of DNA content cell-by-cell and, by extension, cell cycle analysis.

Background
Refer to Smith paper text

Chemical and Fluorescence Properties
DRAQ5 is highly lipophilic, permitting it to rapidly cross plasma and nuclear membranes in eukaryotic cells and tissues. It is readily water soluble making it compatible with typical physiological buffers and media used in cell biology. DRAQ5 is deep blue to the naked eye.

Toxicity
topoisiomerase II inhibitors