User:Herrerais

Peer Review - Marissa Ogata

Overall I think you did an excellent job with this page. I made a very minor grammatical error (added an apostrophe where necessary), but besides that I didn't see many grammatical - if not any - grammatical errors which is good. One thing I think would be important to include is what happens after the immunofluorescent test is complete? Is it possible to cleave the antibodies and the fluorophore back off of the molecule? And if so, what is the chemical mechanism by which this occurs? Also, when photobleaching is mentioned, there is a word-link to another wiki page for photobleaching. However, it would be helpful to give a very brief, one sentence explantation or definition of photobleaching rather than jumping into how to reduce photobleaching without explaining initially what it is. Also, I think the captions for the pictures could be shortened a bit; try to make it a bit more concise. If certain information in the caption is important, consider moving it from the caption into the main body/text where it is relevant.

Peer Review of Immunofluorescence by Kevin

The use of the personal pronoun you needs to be changed and modified in the section of Preparation of Fluoresence. In Primary Immunofluoresence, I think that the cost of the technique might not need to be mentioned. In the second and third paragraph of secondary immunofluoresence, there are no citation and an example is provided in paragraph 3. You might want to consider cutting paragraph 3 out and finding a source to support what is written in paragraph 2. In the third sentence of 1st paragraph of Limitations, you should consider another way to start the sentence because their is vague. The second cited source in bibliography should not be used, as it does not seem to be a reputable source. Overall, I think that the whole section flows very well, is organized clearly and distributed evenly, and the writing is clear.

My Article:

https://en.wikipedia.org/wiki/Immunofluorescence

Direction of edits:

I want to go into the limitations and advancements made in immunofluorescence. I want to add more detail about the method and how the antibodies are made for this technique to work.

Sample edit:

Intact antibodies can also be too large to dye cancer cells in vivo.  Their size results in slow tumor penetration and long circulating half life. Research has been done investigating the use of diabodies to get around this limitation.

My References: