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We can also measure the concentration of enzyme using the property of absorbance. Spectrophotometry is a technique in which we measure the absorbance of the sample, and from this absorbance using Beer–Lambert law we can calculate the concentration of our enzyme by using the following equation.

A = ɛlc

A is absorbance of the sample, ɛ is absorptivity coefficient, l is the length of light traveled in cm, and c is the concentration. From this equation we can see that concentration and absorbance are directly related. in other words, the absorbance will be higher for the sample with higher Protein (enzyme) concentration.