User:Jessicaniezwicki/Ideonella sakaiensis

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Ideonella sakaiensis is a bacterium from the genus Ideonella and family Comamonadaceae capable of breaking down and consuming the plastic polyethylene terephthalate (PET) using it as both a as a sole carbon and energy source. The bacterium was originally isolated from a sediment sample taken outside of a plastic bottle recycling facility in Sakai city   Sakai, Japan.

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Discovery Section

 'Ideonella sakaiensis' was first identified in 2016 by a team of researchers led by Kohei Oda of Kyoto Institute of Technology and Kenji Miyamoto of Keio University after collecting a sample of PET-contaminated sediment at a near a plastic bottle recycling facility located in Sakai city, Japan in Japan. The bacteria bacterium was isolated from a consortium of microorganisms in the sediment sample, that included including protozoa and yeast-like cells. The entire microbial community was shown to mineralize 75% of the degraded PET into carbon dioxide once it had been initially degraded and assimilated by 'Ideonella sakaiensis'


 * Make sure to add link to BHET within the degradation section
 * Delete link to mineralize and assimilated

Characteristics Section

Physical Attributes

Ideonella sakaiensis is Gram-negative, aerobic, and rod-shaped. Cells are motile and have a single flagellum. Colonies of I. sakaiensis are colorless, smooth, and circular. Its size varies from 0.6 to 0.8 μm in width and 1.2-1.5 μm in length as shown below. Chemical Attributes

I. sakaiensis tests positive for containing oxidase and catalase. *tag the paper* The bacterium grows at a pH range of 5.5 to 9.0 (optimally at 7 to 7.5) and a temperature of 15–42 °C (optimally at 30–37 °C).

Use of Characteristics

The bacterium was shown to grow on PET surfaces in a community with other I. sakaiensis cells   by adhering to the PET plastic and other cells with its thin flagellum/appendages. These appendages may also function to secrete PET-degrading enzymes onto the PET surface.