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Multiview light sheet microscopy, also called multiview selective-plane illumination microscope (MuVi-SPIMIM), is a light sheet technology that utilizes more than one illumination or detection arms to realize imaging samples from different perspective. Typically, multi-view microscopy can be used to diminish the loss of signal induced by the absorption or the scattering effects, thus this technology enables higher axial resolution and bigger field of view. The common multi-view light sheet microscopy includes two illumination one detection, one illumination two detection, and two illumination two detection arms.

Advantages
Compared to mono-view imaging without spinning, the imaging quality of which is not high due to extinction, the multiview imaging contains more spatial information of the sample. Compared to mono-view imaging with spinning, the multi-view imaging reduces the needs to spin the sample, thus it is quicker, considering the ratio of time of mechanical spinning and time of imaging is high. Multiview light-sheet microscopy eliminates the need for specimen-specific data fusion algorithms, streamlines image post-processing, and thereby eases data handling and storage.

Principles
If the sample is absorber and two illumination arms are used, the two colliding beams can compensate so that the image will not be dim on one side due to absorption of light. If two illumination and two detection arms are used, by setting different open time for two illumination arms, the 2-2 illu-detection can enable four imaging branches. By fusing the 4 branches, the spatial resolution will be higher as the two symmetrical illumination sources decrease the extinction of signal on the sheet plane when the sheet travels inside the sample.