User:Jmudukes88/sandbox

This is my sandbox.

Tom

Use the links associated w/ this to help build, edit a sandbox page: http://www.youtube.com/watch?v=pAy_kBBqs0U

Five Pillars Summary
The Five Pillars of Wikipedia, here
 * 1) Wikipedia is an encyclopedia made by it's users
 * 2) Wikipedia does not hold any political, religious, or any type of bias
 * 3) Wikipedia is free to use!
 * 4) All interactions with Wiki users should be friendly
 * 5) There are no rules!

Summary Of Characteristics Of Target Article
Good articles are:
 * 1. Well-written:
 * a. respects copyright laws
 * b. correct spelling and grammer
 * 2. Verifiable with no original research:
 * a. contains all references
 * b. provides the correct citations in-text
 * 3. Broad Coverage:
 * a. covers material and does not stray from topic
 * 4. Neutral:
 * a. It offers unbiased viewpoints
 * 5. Stable:
 * a. NO editing wars
 * 6. Well-illustrated with images:
 * a. Images are relevant
 * b. Images are referenced correctly

Using Citation Practice
Telomeres are found at the ends two ends of linear chromosomes. The ends of chromosomes are packed with telomeric proteins that distinguish the ends of the chromosome in order to protect the DNA molecule from degradation or DNA repair activities. The length of telomeres have been discussed as a potential biomarker of aging and general health status. Studies have associated shorter telomeres with cancer and other age-related diseases. For example, idiopathic pulmonary fibrosis (IPF) is the most common disease related to telomere-shortening.

History

 * First DNA based genome to be sequenced by Frederick Sanger in 1977
 * Sequenced by making genomic library
 * Link to Phi X 174?

Genomic Library Construction
http://commons.wikimedia.org/wiki/File:Genomic_Library.png
 * Gives details about BAC vectors and how to construct a BAC library.
 * And also, our class textbook once again
 * http://www.accessexcellence.org/RC/VL/GG/ecb/ecb_images/10_23_genomic_library.jpg
 * http://media-1.web.britannica.com/eb-media/46/117846-004-DE213A57.jpg
 * are either of these pictures suitable and available?

http://commons.wikimedia.org/wiki/File:Plasmid_replication_(english).svg

Types Of Vectors Used
Link to vector? Image retrieved from wikipedia commons. http://commons.wikimedia.org/wiki/File:Genomic_Library.png
 * Genetics: From Genes to Genomes has a chart of different kinds of vectors used for genomic libraries and what size inserts each can hold.
 * Molecular Cloning describes each kind of vector used for genomic libraries in detail (Cosmid, P1, PAC, BAC, and YAC).

How To Select Vector??

 * Displays mathematical model of how to select vector (how many clones needed to represent library and at what confidence level)
 * Details specific vectors, size, advantages/disadvantages, and examples
 * Provides key principles of vector selection
 * Great, detailed overview of Genomic library creation
 * Is this referenced appropriately?
 * Genetics: From Genes to Genomes also explains some pros and cons to vectors and factors affecting the decision of which vector to use. It mentions too how to determine how many clones will be needed for coverage of a whole genome, but does not go into detail about calculations for coverage/confidence level.

Applications
Provide examples of these:
 * genetic engineering
 * genetic mapping and analysis in different organisms
 * analysis of healthy and diseased, etc.
 * The book A Primer of Genome Science covers the use of genomic libraries for hierarchical sequencing.
 * BAC libraries had a role in sequencing the human genome.
 * BAC libraries can be made with small fragments from bacterial genomic DNA in order to study gene function of bacteria that can't be cultured easily (pathogens)