User:Jnp15c/sandbox

In the laboratory, the strength of this interaction can be measured by finding the temperature necessary to break the hydrogen bonds, their melting temperature (also called Tm value). "For a sequence of 14 nucleotides or less Tm (degrees C) = (2*A+T)+(4*G+C) is used to calculate melting temperature. If the sequence is greater than 13 nucleotides Tm (degrees C) = 64.9+41*(G+C-16.4)/(A+T+G+C) is used to calculate melting temperature." When all the base pairs in a DNA double helix melt, the strands separate and exist in solution as two entirely independent molecules.