User:Jschnetzer/Sandbox

The Ocean Sampling Day(OSD) is massive sampling campaign of researchers around the world and is coordinated by the MicroB3 community. It will be conducted on the summer solstice (June 21st) in the year 2014. The OSD will involve all Micro B3 partners with study sites and any interested lab across Europe and beyond. These cumulative samples, fixed in time and space supplemented with a broad set of geo-referenced environmental parameters, will contribute to determine a baseline of marine biodiversity and functions on the molecular level.

Pilot OSD (21.6.2012)

 * Max Planck Institute for marine microbiology, Germany
 * UC Berkeley’s Gump Station, Moorea
 * CNRS-EPHE CRIOBE, Moorea
 * Mer-Station Biologique de Roscoff, France
 * Rothera, British Antarctic Survey research station
 * Matis, Iceland
 * Blanes Bay Microbial Observatory,Spain
 * CIESM, France
 * VLIZ, Belgium
 * USC, USA
 * Biologische Anstalt Helgoland, Germany
 * HCMR, Greek

Sampling
To ensure maximum usefulness of these samples, sampling and data analysis will be done across all sites using agreed upon best practices developed within Micro B3. With this a high level of consistency between data points across Europe and beyond is ensured. In particular all sites will be expected to comply to the minimum information checklists of the Genomic Standards Consortium for describing ‘Omics samples. One liter of water will be filtered using Sterivex 0.2um to collect the Microbial community. Additional water samples will be used to investigate environmental parameters like temperature, salinity, pH, phosphate-,ammonium, nitrate and nitrite concentration as well as others depending on the sampling side.

Sequencing
For the Pilot OSDs the sequencing will be done by the Earth Microbiome Project following their protocols.

Crowfunding
References: http://www.microb3.eu/work-packages/wp2 http://oceansamplingday.blogspot.de/ http://www.microb3.eu/home