User:JustinCHEM446/Outline

Protein Adsorption

Trevor Shepherd, Justin Hui, Jessica Cho

Methods to investigate protein adsorption
1. Fluorescent labeling / Radio labeling

2. Atomic Force Microscopy (AFM)
 * Measures the force of adhesion between a protein-coated probe and the arrayed polymers.

3. Ceramics: 2D PAGE

4. Surface-Matrix Assisted Laser Desorption Ionization Mass Spectrometry (Surface-MALDIMS)
 * Instrument uses a pulsed UV laser for protein ionization and delayed extraction combined with time-of-flight analysis for mass separation

Relevance
1. Sterilization of implants and medical devices (reduced risk of disease transmission
 * a. Surgical tool design

2. Reduced risk of corrosion

3. Engineer devices to have a lower risk of rejection after implantation
 * a. Incorporating native proteins will reduce the host immune and inflammatory response

Chemical composition
1. Crystalline structure
 * a. Crystal packing exposes potential metal-ion-dependent adhesion site (MIDAS) on one edge of the beta-sandwich and may serve as a binding site for collagens and other ligands.

IMFs
1. Dispersion

2. Dipole-dipole

3. Hydrogen Bonding

Surface wettability
1. Contact angle

2. Surface energy