User:Kaitlynwu18/Termination signal/Alib2022 Peer Review

General info

 * Whose work are you reviewing?

Kaitlynwu18


 * Link to draft you're reviewing
 * https://en.wikipedia.org/wiki/User:Kaitlynwu18/Termination_signal?veaction=edit&preload=Template%3ADashboard.wikiedu.org_draft_template


 * Link to the current version of the article (if it exists)
 * Termination signal

Evaluate the drafted changes
(Compose a detailed peer review here, considering each of the key aspects listed above if it is relevant. Consider the guiding questions, and check out the examples of what feedback looks like.)

Lead

"A termination signal is a sequence that signals the end of transcription or translation." Good and clear definition.

"It is needed because only gene-encoding parts of the chromosome should be transcribed. Termination signals bring a stop to transcription, ensuring that genes are properly transcribed." This seems a little repetitive, maybe find a way to merge the two sentences?

"Transcription begins at the promoter when RNA polymerase, an enzyme that facilitates transcription of DNA into mRNA, binds to a promoter, unwinds the helical structure of the DNA, and uses the single-stranded DNA as a template to synthesize RNA." This sentence is a little confusing to read for me, maybe arrange it a little differently (like making it two sentences or use the commas differently). Or maybe define DNA polymerase before the sentence so that it does not take away from what you are trying to say about transcription.

For the lead section, maybe add a brief description of the other sections (this is based on the peer review prompt).

Polymerase Read-Through

"Termination signals cause the release of RNA polymerase from the growing RNA transcript, ensuring that transcription only occurs for parts of the DNA sequence that are meant to be expressed by the cell. Termination signals are important aspects of gene regulation because they mark the end of a gene transcript. Thus, inhibition or activation of termination can change expression levels of certain genes." Maybe ask our TA for advice on this but I don't know if you have to cite the article after each sentence if the whole paragraph uses the same reference.

"Antitermination involves the inhibition of terminators, thus preventing RNA polymerase from detaching from the RNA in response to a termination signal and increasing downstream gene expression" Maybe can improve wording here? The part I bolded seems a little out of place?

"Two types of termination signals in bacteria are intrinsic and factor-dependent terminators. Intrinsic termination occurs when a specific sequence on the RNA elicits detachment of RNA polymerase from the RNA-DNA complex. In E. coli, one termination signal consists of an RNA hairpin that has high amounts of guanine and cytosine as well as a region high in uracil nucleobases. Factor-dependent terminators require proteins for proper termination. Rho-dependent termination is a common termination mechanism found in bacteria that involves the binding of Rho protein to remove RNA polymerase from the DNA-RNA complex. " I like the definition and examples for the two types of termination signals.

I like the information on antitermination and how it can be important.

Termination Signal Efficiency

"Termination efficiency of T7 RNA polymerase is around 74%, which creates issues when T7 RNA polymerase is used to produce recombinant proteins." What is the T7 RNA and why is it important to discuss? (ie why did you choose this one to talk about?) Is there more general information about this?

"Selectable marker genes that are downstream of the target gene insertion site and genes that encode regulatory proteins may have altered expression as a result. Hence, proper termination of transcription is needed for plasmid stability in host cells for the proper production of recombinant proteins." Good explanation of the importance of proper termination of transcription.

Translational Termination Signals

No content here? Did you forget to add this or remove the section?

Overall, I think it is a good introduction into termination signals. I also liked the pictures that you used. Most of what I caught were just areas where the wording or grammar can be improved. If you are looking for more ideas on content that you can add, maybe have a section on application or why we care about being able to understand it. I think that your additions will definitely add to the current article. Nice job!