User:Kcq12/sandbox

Getting used to the sandbox.

Article Glucan

Info present

- explanation of structure (linkage)

-mention of where prevalence occurs

-mention of application

-Subsections of known glucans

-A Link to B-glucan Wiki (which is beyond stub level-)

[Note to self: don't add too much about B-glucan as it has its own page]

Where to add

- Current extraction

- More on alpha

-where are they present

-glucans in fungi as example of their role

-applications in treatment(?)

"Current Applications" [Header]

alpha

alpha in fungi: connected to pathogenicity +virulence


 * protects invading fungus from being recognized by host during infection (covers the surface of the fungal cell wall

beta


 * the ability to alter chain length and functional groups within B-glucan to promote activity
 * Structure, preparation, modification, and bioactivities of β-glucan and mannan from yeast cell wall: A review
 * what these biological activities are (5) and how B-glucan can be changed to achieve this (4)

Mechanisms (of synthesis)

enzymes used

Edits

Sources

β-glucan recognition by the innate immune system

DOI: 10.1111/j.1600-065X.2009.00793.x

Structure, preparation, modification, and bioactivities of β-glucan and mannan from yeast cell wall: A review

https://doi.org/10.1016/j.ijbiomac.2021.01.125

'''Cell wall glucans of fungi. A review'''

https://doi.org/10.1016/j.tcsw.2019.100022

^^Acceptable secondary medical/scientific sources^^

- Update: Add in "Structure and Function of a-glucan debranching enzymes" (Moller, Henriksen, Svensson)

Properties

-water-solubilty (membrane glucans insoluble, storage glucans soluble+ in cytoplasm)


 * Glucans found in organelles, cytoplasmic space, cell wall, where else... (to avoid making incorrect generalizations)

[Other physical/chemical properties]

New Section: Function

As energy stores (but say in another way or its plagiarism)

In the cell wall of fungi,


 * alpha glucans
 * do not appear to be essential in some species
 * In other species,
 * immunoevasion! Covers the cell wall surface so host does not recognize it! Protects "invading hyphae" from being digested by host enzymes!
 * improves virulence of pathogenic fungi
 * (without a-1,3 glucans, a decrease in virulence was observed)
 * beta glucans
 * fortify cell wall
 * "architecture and resistance of the cell wall"
 * when these polysaccharides are digested or are not allowed to be synthesized (not present), cell lysis occurs or abnormal cellular morphology is seen (morphology describes form/structure)
 * host recognition (which is bad for the fungi)

Interesting vocabulary

Pathogen-associated molecular patterns (PAMPs)

Psuedonigeran (a-1,3 glucans)

Notes on state of article

Leading sentences in the opening seem isolated= could add a sentence or two in order to improve flow of introduction (further orient the reader, make next sentences more relevant)

I would switch structure to be immediately after the examples- properties and functions tend to come about from the structure

(+ also add more on structure? Linear or branched, amorphous or structured microfibrils- but need acceptable sources that explain that)

"They represent a drug target for antifungal medications of the echinocandin class."

What drug exactly? Where is the source of this (of medications, of mechanism) I would like to find info on this rather than remove it (it is likely true but needs to be accompanied by a source!)

Structure section is extremely well informed...but seems like it is from an entirely separate article (Update: which it was; it was later merged into this as is)

More info on what unhydrolysed polymers are (???) Radical R (?)

I feel like they are just naming very specific ideas, and they are not commonly known (I had to look up every third word...)

I like the addition of images! And the solid amount of existing references

I am not sure if template Reflist has been added or not.

Future Additions

Laboratory Techniques!