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Mechanism of action
In pharmacology, the term mechanism of action (MOA) refers to the specific biochemical interaction through which a drug substance produces its pharmacological effect. A mechanism of action usually includes mention of the specific molecular targets to which the drug binds, such as an enzyme or receptor. Receptor sites have specific affinities for drugs based on the chemical structure of the drug, as well as the specific action that occurs there. Drugs that do not bind to receptors produce their corresponding therapeutic effect by simply interacting with chemical or physical properties in the body. Common examples of drugs that work in this way are antacids and laxatives.

Drugs with known mechanisms of actions
There are many drugs in which the mechanism of action is known. By knowing the interaction between a certain sight of a drug and a receptor, other drugs can be formulated in a way that replicates this interaction, thus producing the same therapeutic effects. Indeed, this method is used to create new drugs.

Aspirin
The mechanism of action of aspirin involves irreversible inhibition of the enzyme cyclooxygenase; therefore suppressing the production of prostaglandins and thromboxanes, thus reducing pain and inflammation. This mechanism of action is specific to aspirin, and is not constant for all nonsteriodal anti-inflammatory drugs (NSAIDs). Rather, aspirin is the only NSAID that irreversibly inhibits COX-1.

Antidepressants
The range of pharmacology of antidepressants is broad, as there are many different mechanisms of actions of antidepressants. Many antidepressant drugs, including citalopram, fluoxetine, and paroxetine, serve as selective serotonin reuptake inhibitors (SSRIs). The mechanism of action of SSRIs involves the blockage of the serotonin transporter (SERT), which prevents the reuptake of serotonin. An increased level of serotonin in then present in the synaptic space, leading to repeated stimulation of receptors in the recipient cell, thus, producing its pharmacological effects.

Drugs with unknown mechanisms of action
Some drug mechanisms of action are still unknown. For example, phenytoin is used to treat symptoms of epileptic seizures, but the mechanism by which this is achieved is still unknown, despite the use of the drug for many years. . However, even though the mechanism of action of a certain drug is unknown, the drug still functions, it is just unknown or unclear how the drug interacts with receptors and produces its therapeutic effect.
 * Acamprosate
 * Armodafinil
 * Cyclobenzaprine
 * Demeclocycline
 * Fabomotizole
 * Lithium
 * Meprobamate
 * Methocarbamol
 * Paracetamol
 * Phenytoin

Mechanism of action vs. mode of action
In some literature articles, the term mechanism of action and mode of action (MoA) are used interchangeably; typically referring to the way in which the drug interacts and produces a medical effect. However, in actuality, a mode of action (MoA) describes functional or anatomical changes, at the cellular level, resulting from the exposure of a living organism to a substance. . This differs from a mechanism of action, as it is a more specific term that focuses on the interaction between the drug itself and an enzyme or receptor and its particular form of interaction, whether through inhibition, activation, agonism, or antagonism. Furthermore, the term mechanism of action is the main term that is primarily used in pharmacology, whereas mode of action will more often appear in the field of microbiology or certain aspects of biology.

Direct biochemical methods
Direct biochemical methods include methods in which a protein or a small molecule, such as a drug candidate, is labeled and is traced throughout the body. This proves to be the most direct approach to find target protein that will bind to small targets of interest, such as a basic representation of a drug outline, in order to identify the pharmacophore of the drug. Due to the physical interactions between the labeled molecule and a protein, biochemical methods can be used to determine the toxicity, efficacy, and the mechanism of action of the drug.

Computation inference methods
Typically, computation inference methods are primarily used to predict protein targets for small molecule drugs based off of computer based pattern recognition. However, this method could also be used for finding new targets for existing or newly developed drugs. By identifying the pharmacophore of the drug molecule, the profiling method of pattern recognition can be carried out where a new target is identified. This provides an insight at a possible mechanism of action, as it is known what certain functional components of the drug are responsible for interacting with a certain area on a protein, thus, leading to a therapeutic effect.