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Ropalidia romandi  is a species of paper wasp that is found in Northern and Eastern Australia. It is also known as the yellow brown paper wasp or the yellow paper wasp. R. romandi is a swarm-founding wasp, and manages perennial nests. Its nests are known as 'paper bag nests' and they can have very different architecture depending on the substrate on which it is built. It can often have multiple strepsipteran endoparasites, and it delivers a very painful sting when threatened.

Taxonomy and phylogeny
Ropalidia romandi, first named by Le Guillou in 1841, is in the subfamily Polistinae. It is in the genus Ropalidia, which is the only genus in Polistinae that contains both independent-founding and swarm-founding species. This means it must have evolved swarm founding independently from other swarm-founding polistine genera. Ropalidia is part of a monophyletic group with the other three Old World polistine genera, and contains approximately 180 species. This genus is endemic to Oceania and the Old World. Ropalidia romandi contains two subspecies Ropalidia romandi romandi Le Guillou 1841 and Ropalidia romandi cabeti de Saussure 1853.

Description
Ropalidia romandi is unique for its overall yellow color, with dark brown thorax and abdominal markings. It is quite small and has been measured to be only around 12mm. The first segment of the abdomen in Ropalidia is narrower than the following segments and because it is a paper wasp, their front wings fold lengthwise when resting.

Its nests are referred to as ‘paper bag nests’ because it consists of an papery envelope covering several horizontal combs. Both the envelope and its contents are made of papery plant fibers glued together with saliva. The nest is either spherical or hemispherical in shape, can be up to one meter long and are usually in trees or eaves of buildings, a number of meters off the ground.

Distribution and habitat
R. romandi is found in Australia, in Northern Territory and Queensland. It is one of the most common species in Eastern Queensland. R. romandi lives in both tropical and temperate climates, however their habitats are prone to cold winters, which impacts its seasonal colony activity. Nests can be found hanging from the underside of various tree branches and building eaves at anywhere from 2.5m to 30m above the ground. They commonly attach their nests to Eucalypts, which shed their bark frequently. When this occurs, the nests fall to the ground, are abandoned by the colony and a new nest is constructed.

Colony Cycle
R. romandi is a swarm-founding species, which means their colonies are founded by a swarm of workers associating with queens (fertilized females). This is in contrast to what happens in independent-founding species, where the colony is founded by only one or a few fertilized females. R. romandi has been found to manage perennial nests, which can last at least a few years. This means that the colony does not die off in the winter as they do in annual colonies, but they instead survive the winter and manage the same nest the following season. Because of this, swarm-founding species normally have larger colonies with more queens than independent-founding species. Colony survival over the winter is thought to be possible because R. romandi practices seasonal colony activity. This means that in summer, the wasps are more active, performing active reproduction, while in the winter the number of reproductives die off, and no reproductive activity is observed.

There is also a difference in colony foraging behavior between the winter and summer seasons. Workers can be seen foraging for flower nectar (which has lots of carbohydrates) much more frequently in the winter than in the summer, suggesting that the wasps use these carbohydrates as energy stores to survive the winter. These energy stores make it possible for the wasps to not take any active flights for up to a week, which is beneficial because they avoid the cool temperatures and often rainy weather.

During R. romandi 's colony cycle, there is a large difference in the composition of the cells within the nest. Winter nests have more nectar deposits in cells than larva. In contrast, summer nests had only 1% of their cells filled with nectar, while the rest contain developing immatures. This again points to the seasonal colony activity of R. romandi with more reproductive activity, and therefore brood raising, occurring in the summer, and more foraging behavior and nectar storing occurring the winter.

(Typically more males in the colony than females??-original). there are multiple queens, workers and males found in R. romandi colonies.

Swarm-founding
The genus Ropalidia is unique because it contains both independent and swarm-founding species. R. romandi is a swarm founding species, meaning that new nests are founded by a large group of workers with a smaller number of inseminated females (egg-laying queens). There are two different types of swarms: reproductive and absconding. A reproductive swarm is made up of some females of an already established colony, who depart to form a new colony with a group of workers. Absconding swarms, on the other hand, are made up of all the members of a colony and is the continuation of the existing colony. R. romandi is thought to utilize both forms of swarming, however, not much is known about reproductive swarming in Ropalidia  and most research on R. romandi has been on absconding swarms. It has been found that R. romandi will abscond if their nest is destroyed, is damaged, or if it is continually disturbed.

Absconding Process
During the absconding process, it is the workers, and not the queen, as it is in independent founding species, that choose the new nesting site. It has been hypothesized that the process is mediated by pheromones. During their search for a new nesting site, R. romandi workers can be observed exhibiting a behavior called ‘metasomal dragging’. This means that the wasp drags their metasome along objects on which they land. These objects are usually leaves, rocks, or man made structures. There are glands on the underside of the gaster that leave a scent behind for the other workers and the queens to follow. Wasps that didn't exhibit metasomal dragging were often seen inspecting objects with their antennae, presumably following the scent trail. This pheromone path helps recruit all other members of the colony to the new nesting site. Visual information may also be very important during the R. romandi absconding process, and may even be the ultimate sign of the new nesting site. As wasps continue to make their way to the new nesting site, they can be seen orienting themselves to aerial swarms that had formed around the new site. This orientation to aerial swarms was seen even in the absence of continued metasomal dragging, so it has been hypothesized that the visual cue of the aerial swarm is the indication of the final nesting site.

Aggressiveness during swarming
R. romandi, along with other swarm-founding wasps, can be quite aggressive during the swarming process. They are aggressive towards humans, non-nestmates, and to each other. Aggressiveness towards humans may result in stings, while aggressiveness towards other wasps involves biting of the body, legs or wings. Workers are more often the aggressors, while queens are most often the victims. What is interesting about R. romandi, is that workers do not always act out against non-nestmates. If different colonies of R. romandi are introduced to each other during the absconding process, they will cluster together and relatively little violence is exhibitied against non-nestmate queens at this stage. If a queen is introduced after new colony formation, however, she will be vigorously attacked. It has been hypothesized that this means that R. romandi workers test out different queens during the absconding process.

Genetic relatedness in colonies
Because swarm-founding colonies can contain more than one egg-laying queen, they tend to challenge the kin selection theory and typically do not meet Hamilton’s ¾ rule of haplodiploid species. In comparison to other swarm-founding species, these theories are challenged further in R. romandi. R. romandi colonies are extremely large, and can have an average of up to 400 queens at any one time. The average for other swarm-founding species is only around 20 queens. Having such a large number of queens decreases the genetic relatedness within the nest. Workers in R. romandi have a genetic relatedness of only 0.16 and queens have a genetic relatedness of only 0.34. The next lowest genetic relatedness among a swarm founding species is 0.24 among workers and 0.55 among queens and this was found in Polybia emaciata  ''. ''These numbers indicate a large deviation from Hamilton’s ¾ rule. Independent-founding species, with fewer queens, are much more likely to be more genetically related.

It has also been suggested that R. romandi practices cyclical oligogyny, or the phenomenon that new queens are made when the number of queens is reduced. This was hypothesized because a difference was found between the number of effective queens in a colony, and the mean number of queens.

Morphological caste differences
It has been found that R. romandi queens and workers differ in size and body shape, however these differences vary between colonies and are sometimes insignificant. If worker-queen dimorphism is present, the largest observed difference is between the metasome, with queens having larger metasoma, and longer and more reddish second metasomal segments. The most significant difference between queens and workers is between the developmental condition of their ovaries. Queens had ovaries in which all ovarioles had mature or semi mature oocytes, and they were showing sequential development of oocytes. Workers, on the other hand, had ovarioles that were filamentous or had only partially developed oocytes. Some individuals had ovaries that were between the queen and worker stages. These wasps are thought to be workers that become egg laying during swarming events, or they may by young uninseminated queens.

Parasites
A common endoparasite of R. romandi are insects of the order Strepsiperans, commonly in the family Stylopidae. Strepsipteran females are permanent endoparasites that dissolve through the cuticle of the host as larvae, create a sac from the host tissue to protect themselves from host defenses, and then reside in the abdomen of the host, taking up 80% of the volume of the abdomen. These parasites can cause sterility, sometimes causing ovaries and spermathecae to become unrecognizable, and often change the host morphology and behavior. However, they do not kill their host, and it has even been suggested that they can prolong their hosts life. R. romandi often has multiple stylopisation, meaning they have more than one endoparasite at a time.

Addtionally, R. romandi broods can be attacked by hornets. The hornets take larvae from the nest, sometimes over a period of just days, sometimes over weeks. It has been hypothesized that the envelope of a nest functions to reduce exposure to parasites.

Nest development
R. romandi nests are referred to as ‘paper bag nests’ because it consists of an papery envelope covering several horizontal combs, all of which is made of papery plant fibers glued together with saliva. The nests can be up to one meter long and are usually in trees or eaves of buildings, a number of meters off the ground. To begin building a nest, a number of wasps gather at the nesting site, and begin building several combs simultaneously. This simultaneous building leads to semispiral shape within the nest because irregular connections are made when the combs are connected at their growth fronts. The envelope is either constructed after the construction of the first few combs or during that process, and the timing of envelope construction depends on swarm size, and nest location. More combs are then added, and the envelope is then completed to cover all combs. The number of entrance holes depends on nest size. R. romandi never builds double sided combs and the number of cells in each comb varies. The cells are all of similar size, which reflects the small and sometimes insignificant size dimorphism that is present in some colonies of R. romandi .

Solid surface nest architecture
R. romandi nests are often built under the eaves of buildings. When built on such a hard surface, the nest is shaped as a compressed hemisphere, using the eave as its top. It has multiple horizontal, flat combs, with the largest comb being near the bottom. These combs are connected to each other by pedicles, which have been described as ribbon like. Cells are hexagonal and regularly arranged. The nest is entirely covered by an envelope. The envelope is mostly single layered, however it can be double layered in parts. It is made of plant fibers and fragments and is a uniform light gray color. It is possible for the envelope to be so heavily coated in saliva that the fibrous material of the envelope itself is completely hidden. A round entrance hole is at the bottom of the hemispherical nest.

Variable surface nest architecture
Nests are also found built among the branches of various species of trees. When this occurs, the architecture of the nest is changed because of the substrate to which it has to attach. The most noticeable difference is that R. romandi nests connected to branches are shaped as a sphere as opposed to a hemisphere. Addtionally, the envelope is mostly double layered, with only a few single layered sections. The envelope is not a gray color, but instead a creamy brown, suggesting differences in the plants available to construct the nest. The saliva coating of the envelope is thicker when built in trees, suggesting that the thickness of the coat might be related to the rain exposure of the nest location. Instead of a roof, the nests often use leaves from the tree, incorporated into the envelope, to cover the top of the nest. The number of combs in a branch built nest is almost double that of one built on a solid surface, however they are much more irregular in shape and arrangement.

Human importance
While all wasp stings are unpleasant, the sting of R. romandi is known for being very painful. Additionally, because R. romandi  is a paper wasp, it has the ability to repeatedly sting without dying. R. romandi is most aggressive if they are swarming, if their nests are disturbed or if the wasps feel their nest is threatened. If wasps feel threatened, they may swarm out of the nest, and pursue the aggressor. If you do ever get swarmed by wasps, it is recommended that you remain as still as possible and move away slowly. Sudden movements will be regarded as aggressive acts and will result in more stings.

If stung, the site will appear red and may remain itchy and swollen for many days. The best treatment of stings is to first wash the area with soap and water, then use an antiseptic, and if swelling remains apply an ice pack. Many people are allergic to wasp venom, and if this is the case, a sting can result in severe anaphylactic shock Symptoms of this include swelling of airways, including mouth lips and tongue, troubled breathing, dizziness and tightness of chest. If an individual exhibits any of these symptoms after acquiring a sting from R. romandi seek medical attention immediately.

Because R. romandi nests can often be found on buildings, it is common for wasp nests to be built in heavily human populated areas. Their nests do not cause any structural damage, so if the nests are located in out of the way area, they are best left alone. Attempting to knock them down will induce the aggressive behavior described above, and R. romandi tend to rebuild nests in similar spots. Additionally, paper wasps feed their young on caterpillar, so they can help eliminate common garden and agricultural pests. If one does have to get rid of a nest, it is recommended that the job is done by a professional exterminator, or with a fast acting can of insecticide. The best time to get rid of an R. romandi nest is at dawn or after dark when the wasps are most docile.

Diet
Paper wasps such as R. romandi are known to feed their young on caterpillars. Additionally, they feed on the nectar of many flowering trees, including Syzygium cormiflorum and Acasic oraria. Flower nectar foraging behavior increases during the winter, and is thought to be due to the fact that the high carbohydrate level of flower nectar allows the colony to survive the winter. R. romandi shows another interesting activity in the winter as well: they are seen foraging for honeydew from scale insects. Addtionally, they have been observed defending and protecting the scale insects against other wasps.