User:Kohw

Did you know
Did you know that one primary transcript of mRNA can produce many different mature mRNAs that code for different protein products?

Possible suggestion to the articles
Polyspermy- Polyspermy article talk page. Suggestion : This article is excellent in general background information and processes involved with polyspermy. However, this article would be better for people to understand the concepts behind polyspermy with less confusion if more details are added to it. For example, pictures, or visual of the steps, regarding the process behind polyspermy, fast, and slow block will provide people with more clear explanations. In addition, additional helpful information can be brought from information provided in NCBI bookshelf (on the text Developmental Biology, 6th edition). The full citation for the information is: Gilbert SF. Developmental Biology. 6th edition. Sunderland (MA): Sinauer Associates; 2000. Gamete Fusion and the Prevention of Polyspermy. Available from: http://www.ncbi.nlm.nih.gov/books/NBK10033/. The information provided by this site gives us better insights on normal gamete fusion. Once this information is provided, information from other web or text can be used to explain what causes inviable zygote in polyspermy. Further, this site provides detailed information on prevention of polyspermy, starting with comparison between monospermy and polyspermy then providing details of fast and slow block. This article provides information of fast and slow block but is not detailed. This site gives details on how sodium and potassium concentration and change in resting membrane potential plays a role in fast block. It also gives details on cortical granule reaction (process of reaction and how calcium affects this reaction) and zona reaction in slow block affects preventing polyspermy. Furthermore, the article can be improved by using information from research article from PubMed that is provided in the NCBI site. The full citation is: van der Ven HH, Al-Hasani S, Diedrich K, Hamerich U, Lehmann F, Krebs D. Polyspermy in in vitro fertilization of human oocytes: frequency and possible causes. Ann N Y Acad Sci. 1985;442:88-95. . It provides information on the research done on polyspermy in in vitro fertilization of human oocytes (frequency and possible causes of polyspermy as well as possible methods to reduce polyspermy). Such research reference could give readers a better understanding on this topic. Other than some potential details that could be added, this article was clear on information provided!

Precursor mRNA- Precursor mRNA article talk page link. Suggestion : This article provides information on explanation of precursor mRNA and how precursor mRNA transcript leads to mature mRNA through processing. However, this article could be improved with more informations added. For example, more detailed information on how precursor mRNA becomes mature mRNA through processing, which can be seen in the bookshelf of NCBI site (on text The Cell: A Molecular Approach. 2nd edition). Full citation is: Cooper GM. The Cell: A Molecular Approach. 2nd edition. Sunderland (MA): Sinauer Associates; 2000. RNA Processing and Turnover. Available from:http://www.ncbi.nlm.nih.gov/books/NBK9864/) The information provided here provides pictures for better understanding and details on RNA processing in eukaryotes as well as prokaryotes and on turnover. In addition, more details regarding processing of precursor mRNA could be added. For example, information regarding the effects of 5' cap and poly A tail addition during processing could be included (how do they effect mature mRNA and proteins encoded later). Further, more details on splicing could provide better understanding of the bigger picture of the process to encode protein from transcription to processing to translation. Details on how alternative nRNA splicing could affect mature mRNA and protein isoforms, on the role of splicing enhancers, and on recognition factors could be added to provide a more clear explanation on the bigger picture as well as importance of the process of turning precursor mRNA into mature mRNA. Furthermore, informations on experiments involving precursor mRNA could possibly be added to show what factors can interfere with processing of precursor mRNA into mature mRNA. For example, a research article on PubMed that is provided by NCBI site shows that FUra could lead to cell death by inhibiting mRNA processing. The full citation is: Will CL, Dolnick BJ. 5-Fluorouracil inhibits dihydrofolate reductase precursor mRNA processing and/or nuclear mRNA stability in methotrexate-resistant KB cells. J Biol Chem. 1989 Dec 15;264(35):21413-21. . With some more information added, this article would provide readers with better understanding on this subject!

Primary transcript- Primary transcript article talk page link. Suggestion : This article only gives information that precursor mRNA as primary transcript becomes mRNA. This article may have many repetitive information with the other wikipedia article on precursor mRNA. However, this article could be improved to give better understanding in primary transcript. First of all, more information on how primary transcript is produced should be added (the process of transcription leading to production of primary transcript). It would help readers to understand the process better if visual of the steps is provided! In addition, informations on how regulations on transcription can affect the production of primary transcript through transcription. Such details may include silencer's job on repressing transcription of a particular gene, the job of transcription factors, and etc. Then, a light explanation on how primary transcript becomes mature mRNA through RNA processing could be added to give insight of a bigger picture. The information above are provided in many web pages and texts. The text Developmental Biology, 10th edition by Scott F. Gilbert provides a clear and detailed explanation on the topics above.