User:LLLui/Cytochemistry

Definition
This is the study of the localization of cellular components through the used of staining methods. Immunocytochemistry is studied by tagging monoclonal antibodies through fluorescents.

Freeze fracture enzyme cytochemistry
Freeze fracture enzyme cytochemistry was initially mentioned in the study of Pinto de silva in 1987. It is a technique that allows the introduction of cytochemistry into a freeze fracture cell membrane. immunocytochemistry is used in this technique to label and visualize the cell membrane's molecules. This technique could be useful in analyzing the ultrastructure of cell membranes. The combination of immunocytochemistry and freeze fracture enzyme technique, research can identify and have a better understanding of the structure and distribution of a cell membrane.

Origin
Jean Brachet's research in Brussel demonstrated the localization and relative abundance between RNA and DNA in the cells of both animals and plants opened up the door into the research of cytochemistry. The work by Moller and Holter in 1976 about endocytosis which discussed the relationship between a cell's structure and function had established the needs of cytochemical research.

Aims
Cytochemical research aims to study individual cells that may contains several cell types within a tissue. It takes a nondestructive approach to study the localization of the cell. By remaining the cell components intact, researcher are able to study the intact cell activity rather than studying an isolated biochemical activity which the result may be influenced by the distorted cell membrane and spatial difference.