User:Langmath/sandbox/Cccp-1

Conserved coiled-coil protein 1 (cccp-1) is a gene identified in Caenorhabditis elegans that codes the protein CCCP-1 on chromosome III: 17.95. The best human ortholog of cccp-1 is CCDC186 (coiled-coil domain containing 186). CCCP-1 shares properties similar to that of golgins.

Coiled coils are supercoils.

Contents

 * 1 Molecular interactions
 * 2 Associated effects at intracellular and behavioral levels
 * 3 Expression and localization
 * 4 Properties
 * 5 Motifs
 * 6 C-terminus
 * 7 Loss of function elegans phenotypes
 * 8 References

Molecular interactions
CCCP-1 tethers onto lipid membranes in response to the presence of RAB 2 (elegans UNC-108) as part of dense-core vesicle generation or maturation. CCCP-1 has predicted interaction with T22C8.3 (which has predicted interaction on nucleic acid). Rab 2 and cccp-1 mutants each have a similar phenotype with issues in dense-core vesicle cargo sorting, and double mutants (having both) aren't any worse implying these act in series or as co-factors in the same pathway.

Associated effects at intracellular and behavioral levels
CCCP-1 is involved in dense core granule maturation, locomotion and functions in regulation of locomotion involved in locomotory behavior; cccp-1 exhibits Rab GTPase binding activity. CCCP-1 also has a caspase recruitment domain (CARD domain) implying a role in apoptosis regulation.

Expression and localization
CCCP-1 is expressed in the spermatheca, nervous system, and the intestine; cccp-1 is localized to the axon, the neuronal cell body and the perinuclear region of cytoplasm. More than 60% of intracellular CCDC186 localizes to the cell nucleus.

Properties
CCCP-1's transcript length is approximately 2400kb with a coding sequence just over 2200kb. There are two splice variants: 734 aa and 743 aa. CCCP-1's (the protein product) estimated mass is 85 kDa.

Motifs

 * hmmpanther: start 6, end 732
 * pfscan: start 674, end 734
 * superfamily: start 93, end 342
 * CARD: start 683, end 743

C-terminus
It has been observed that the c-terminus of conserved coiled coil protein 1 (CC3) is necessary and sufficient to localize to and bind RAB 2. This region also has potential binding properties to lipid membranes. The pfscan and CARD motifs span this region. The n-terminus domain has not been characterized.

Loss of function elegans phenotypes

 * mild uncoordination