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Family with Sequence Similarity 166, member B, or FAM166B, is an uncharacterized protein in humans with unknown structure and function that is encoded by the FAM166B gene.

Location
The FAM166B gene is located on the short arm of chromosome 9 at p13.3. The genomic sequence spans from 35563899 to 35561830 for a total of 2,069 base pairs.

Expression
FAM166 shows highest expression levels in bronchi epithelial cells and fallopian tube glandular cells, and also is moderately expressed in heart and skeletal muscle.

Gene Promoter
The Gene Promoter is 680 bp long and is located from 35563717 to 35564396. It contains a number of Transcription Factor Binding Sites, such as CCATT binding factors, Vertebrate TATA Binding Factors, LEF/TCF, Nuclear Factors 1, and Heat Shock Factors.

Homology
FAM166B is conserved in many species with orthologs present in mammals and distant homologs found in several invertebrates.

Splicing Variants
There are four known splicing variants of the FAM166B transcript. Variant One contains 1,092 bp and six exons. The other three variants are 1,024, 1,046, and 1,056 bp respectively. It is interesting to note that all 4 variants contain the six exons of the FAM166B gene.

General Properties
The FAM166B protein spans 275 amino acids in length with a predicted molecular weight of 30574.71 daltons The estimated isoelectric point (pI) of the protein is 8.38.

Isoforms
There are four known isoforms for FAM166B with isoform 1 spanning 275 amino acids. The other three isoforms range from 216 to 306 amino acids.

Intracellular Location
However, the intracellular location of FAM166B is unknown The average hydrophobicity of the protein is -0.519272, which suggests that it is a soluble protein.

Conservation
FAM166B shows high conservation of sequence with its mammalian orthologs. Conservation reduces drastically when reaching Amphibians and Reptiles with % identities from 57% to 26%. Several Invertebrates also show distant homology to FAM166B, but conservation is very low with no sequence identity greater than 30%.

Conserved Domains
FAM166B has three conserved domains, each considered DUF2475 without known functions. The three DUF2475 regions are located from amino acids 15 to 80, 174 to 234, and 234 to 261 within the protein.

Function within Cells
Currently, the function of FAM166B is unknown.

Clinical Significance
There are no current clinical associations of FAM166B with a human disease or condition. It was excluded from two studies concerning a recessive form of hereditary sporadic paraplegia and predicting Hodgkin Lymphoma prognosis due to not meeting proper criteria in both.