User:Lazizjonm/sandbox

Acrasin is a general term for a chemical substance that causes cells to aggregate, or gather. This experiment is to collect and purify a sample of Glorin, which is the acrasin of the P. violaceum species.

GLORIN COLLECTION: P. violaceum were grown and washed in a 1% salt solution. They were then diluted in a 50% salt solution, and 15 mL samples of this solution were placed on petri dishes to allow the P. violaceum to grow and aggregate. After fully aggregated, 40% ethanol was poured over each Petri dish to inhibit acrasinase, and the ethanol was collected and concentrated. (Acrasinase would kill the glorin, so ethanol was needed to inhibit it).

PURIFICATION OF GLORIN: Extract was filtered with water to remove acidic impurities. Whatever was filtered out was mixed with ethanol, and then centrifuged and dried to yield the product. The product was then dissolved in 50% ethanol to prepare for column chromatography.