User:Littleblackraincloud/New sandbox

Biosynthesis
Anatoxin-a is produced by multiple genera of cyanobacteria. It

Detection
HPLC/FD (high performance liquid chromatography with fluorescence detection)

LC-MS/MS (liquid chromatography tandem mass spectrometry

ELISA (enzyme-linked immunosorbent assay)

PPIA (protein phosphatase inhibition assay)

Ingestion
Ingesting drinking water or recreational water that is contaminated with anatoxin-a

Dermal
Dermal exposure is the most likely form of contact with cyanotoxins in the environment. The first study looking at in vitro cytotoxic effects of anatoxin-a on human skin cells found that anatoxin-a exerted no effect at 0.1 µg/mL or 1 µg/mL, and a weak toxic effect at 10 µg/mL. The anatoxin-a was isolated from lyophilized Dolichospermum flos-aquae via solid phase extraction and analyzed with high performance liquid chromatography.