User:Lwinkler19/sandbox

'''What Did I do? Paragraph'''

I quite enjoyed learning and synthesizing information regarding nitric oxide signaling. To start, I edited the first sentence to be more clear, while still succinct. I also added a citation from a review to this sentence, since one previously did not exist. Most of the article paragraph was uncited, so following the Wiki guidelines, I added citations from the review and textbook for two sentences in the second paragraph. Next, I added two sentences in the middle of the second paragraph about the enzymatic targets of nitric oxide, citing the class textbook. This enhanced the article by adding specifics of the biological activity of nitric oxide. I also added the process by which the signal decomposes into nitrites and nitrates because there was information on how nitric oxide was produced but not how it was decomposed. Next, I broke one large paragraph into two smaller paragraphs, expanding on vasodilation. Because nitric oxide signaling is often said to be related to the mechanism of Viagra, I wanted to add the correct information to that section. I synthesized information from a review on viagra for the last two sentences. In total, I added 5 sentences, restructured for better flow, added citations to 8 sentences (mine and previously written info), and added links to other wikipedia articles. Everything I did was while upholding the values of Wikipedia. The introductory sentence was strengthened. Key gaps were filled. The article follows a logical order: formation of nitric oxide, interactions of nitric oxide, then the decomposition of nitric oxide and common uses. The references strengthened the article because they all came from well referenced reviews a textbook. They strengthened the article by highlighting the mechanisms of action of nitric oxide signalling and how it is leveraged in common drugs. The tone, in my opinion, is neutral and the article balances scientific details to the general understanding of nitric oxide signaling. My additions to this article should improve the article for both the lay person and a fellow scientist who needs a review on the cell signaling properties of nitric oxide. My peer reviewer mentioned that she didn't see my additions to the talk page, however I had added them but I am wondering if there is some sort of privacy setting on the talk page.

Nitric Oxide Biological Uses FINAL

NO is a gaseous signaling molecule. It is a key vertebrate biological messenger, playing a role in a variety of biological processes. It is a known bioproduct in almost all types of organisms, ranging from bacteria to plants, fungi, and animal cells.

Nitric oxide, known as an endothelium-derived relaxing factor (EDRF), is biosynthesized endogenously from L -arginine, oxygen, and NADPH by various nitric oxide synthase (NOS) enzymes. Reduction of inorganic nitrate may also serve to make nitric oxide. One of the main enzymatic targets of nitric oxide is guanylyl cyclase. The binding of nitric oxide to the haem region of the enzyme leads to activation, in the presence of iron. Nitric oxide is highly reactive (having a lifetime of a few seconds), yet diffuses freely across membranes. These attributes make nitric oxide ideal for a transient paracrine (between adjacent cells) and autocrine (within a single cell) signaling molecule. Once nitric oxide is converted to nitrates and nitrites by oxygen and water, cell signaling is deactivated.

The endothelium (inner lining) of blood vessels uses nitric oxide to signal the surrounding smooth muscle to relax, thus resulting in vasodilation and increasing blood flow. Viagra, or the generic: sildenafil, is a common example of a drug that uses that works with the nitric oxide pathway. Viagra does not produce nitric oxide, but inhibits the pathways that counteract the nitric oxide pathway, allowing for the signal to be enhanced, and thus vasodilation.

Article Evaluation

I chose to evaluate the article on adrenaline because I am also studying for the quiz tomorrow. The first paragraph (tagline) is well written. There are not any warning banners on the page and many many sources. I checked the articles talk page and there was not much happening on the talk page. The article was given a B class rating which I would agree with. In looking at the history I saw that edits were made as recent as a couple of days ago but most other edits from the year were made in February.

Possible Article Topics

IP3/DAG- This article does not exist yet, but it is protected and I don't have a high enough access to edit it.

1) Nitric Oxide Chosen Article:


 * Content
 * ranges from industrial uses, synthetic schemes, and biological interests
 * biological applications is a small section and more information could be added about its importance in signaling
 * Tone
 * article seems to have a neutral tone
 * Sources
 * everything in the biological applications came from one source
 * should be more sourcing and more citations for more accurate information
 * Talk Page
 * article is part of WikiProject Cell Signalling, WikiProject Chemicals, and WikiProject Medicine
 * article has a B class rating and high-importance level

2) MAPK


 * Content
 * content seems to be complete or close to it
 * strong section on signalling cascades
 * Tone
 * neutral tone
 * Sources
 * would like to see more citations through the article, especially in the "Signal Cascade" section as well as the "Activation" section (there are only 2)
 * Talk Page
 * article is part of WikiProject: Cell Signalling and WikiProject Cell and Molecular Biology
 * article is a B class rating with mid-importance
 * seems to be more talk about the format of the article than the lack of citations or expanding information

3) Adenyl Cyclase


 * Content
 * the content is generally good but I would like to expansion in the regulatory section as well as an addition of a "cell signalling" section
 * Tone
 * neutral tone
 * Sources
 * there are only 8 sources for the whole article
 * the newest source is 2007
 * I would like to see more sources and citations
 * Talk Page
 * article is part of WikiProject: Cell Signalling and WikiProject Cell and Molecular Biology
 * article is a C class rating with mid-importance
 * the last time someone talked was 2008
 * there is little discussion on this talk page

Adding Citations:

Liu, Hongying, Lingyan Weng and Chi Yang. 2017. "A Review on Nanomaterial-Based Electrochemical Sensors for H2O2, H2S and NO Inside Cells Or Released by Cells." Mikrochimica Acta 184(5):1267-1283 ( http://ezproxy.villanova.edu.ezp1.villanova.edu/login?url=https://search-proquest-com.ezp1.villanova.edu/docview/1925896654?accountid=14853 ).

Victor Pena Ribeiro, Caroline Arruda, Mohamed Abd El-Salam & Jairo Kenupp Bastos (2018)Brazilian medicinal plants with corroborated anti-inflammatory activities: a review,Pharmaceutical Biology, 56:1, 253-268, DOI: 10.1080/13880209.2018.1454480

Cell Signalling by John Hancock Third Edition ISBN13: 9780199232109