User:LyndsayReese/sandbox

Uncharacterized protein C9orf84 is a protein that in humans is encoded by the C9orf84 gene. Uncharacterized protein C9orf84 is also known as C9orf84. C9orf84 is currently poorly understood by the scientific community.

Gene
The chromosomal locus of C9orf84 is 9q31.3. It is located on the negative strand, and it contains 34 exons in humans. There are no aliases for C9orf84.

Homology/Evolution
C9orf84 is the only gene in the human genome with its particular sequence. This gene is found in all vertebrates, including chimpanzees, cows, dogs, mice, rats, and frogs, and some invertebrates. The most distant ortholog detectable by NCBI BLAST is in Nematostella vectensis (starlet sea anemone) .The closest plant ortholog to C9orf84 is the SHOC1 protein in Arabidopsis thaliana. C9orf84 is not very well conserved even among mammals, with sequence identity scored reaching only 60% in less related mammals.

Protein
C9orf84 in humans has 17 isoforms .The primarily used sequence is C9orf84 Isoform 1. This isoform is 1444 aa long, has a predicted molecular weight of 165.190 kDa, and a predicted pI of 5.10.

C9orf84 undergoes several post-translational modifications, including glycosylation and o-linked glycosylation, phosphorylation, and it contains leucine-rich nuclear export signals. The secondary structure of this protein is predicted to be mainly alpha-helices in roughly the first two thirds of the protein, and coils in the last third. It is predicted that this protein is localized in the nucleus.

Expression
C9orf84 is ubiquitously expressed in all most tissues with higher than average expression in the testes, the kidney, the thymus, and the adrenal gland. The promoter for C9orf84 in humans is 639 bp long and overlaps with the 5’ untranslated region of the gene. There are four alternate promoters that promote different transcript variants.

Interactions
Several of the most common and most conserved transcription factor binding sites families that are predicted to be found in C9orf84’s promoter region are ETS1 factors, Ccaat/Enhancer Binding Proteins, and Lymphoid enhancer-binding factor 1. ETS1, Ccaat-enhancer-binding proteins, and Lymphoid enhancer-binding factor 1 are all related to immunity.

C9orf84 has been experimentally determined, through a two hybrid pooling approach, to interact with methionine aminopeptidase, a protein encoded by the maP3 gene in Bacillus anthracis.

Clinical Significance
C9orf84 is highly upregulated in psoriasis patients with lesional skin as opposed to psoriasis patients with non-lesional skin and non-psoriasis patients.