User:M2bioinfo-Orsay/sandbox

SQ1002 is a 191nt small RNA identified in Clostridioides difficile 630 by RNA deep sequencing and confirmed by Northern Blot and RT-PCR. Extremities were confirmed by 5’ and 3’-RACE.

This RNA is found in several C. difficile strains in syntenic positions between a putative acyltransferase gene and a Ferrous iron transport gene. No homologues were found outside C. difficile, although a putative paralogue was found in C. difficile 630, located between a Tryptophanyl-tRNA synthetase gene and a pseudogene.

Due to lack of sequence variation, the proposed secondary structure is not supported by any covariation and should be considered speculative.