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Analysis Method of Benzalkonium Chloride in Medicated Pad by UV Spectrophotometer

Each Medicated Pad Contain BENZALKONIUM CHLORIDE 0.5% w/w (As BENZALKONIUM CHLORIDE SOLUTION I.P.) Reagent :- 0.01 M Hydrochloric Acid Picric acid solution :- Weigh about 100 mg of Picric Acid and 14.5 g of Anhydrous Sodium acetate to a 500 ml volumetric Flask, and dissolve in 400 ml of Water.Add 20 ml Of glacial acetic acid,dilute with water to volume And mix. Test Solution :- Remove the medicated pad from the tape by the help Of forcep.and take about 80 mg of medicated pad in Stoppered test tube and add 20 ml of 0.01 M Hydrochloric acid and shake vigorously for 15 minutes

Std. Praparation :-Weigh accurately about 200 mg of Benzalkonium Chloride solution (50% w/w) or according to sample Dissolve in 100 ml volumetric flask with 0.01 M Hydrochloric acid.take 2 ml of this solution and make Up 100 ml with 0.01 M Hydrochloric acid

Procedure :- Pipet 20 ml of Standard solution and 20 ml of 0.01 M Hydrochloric acid to provide a Blank and take above Test solution as above mentioned method into separate Stoppered test tube.Add 5.0 ml of Picric acid solution And 25.0 ml of Chloroform and shake for 2 minute. Collect the chloroform in other dried test tube add 2.0 g of anhydrous sodium sulphate and shake allow the solution to stand for 10 miniutes. Measure the absorbance both STD/TEST at 405 nm And calculate by comperision

Calculation :-

Test Reading x Std Wt. x 2 x 20 x Purity of Std x 100 --                       Std Reading x 100 x 100 Test Wt. x 100