User:MacGyverMagic/Discussions/Lipoproteins

Wnt and (apo)lipoproteins
I'm trying to find info on the following questions in scientific articles, but the specific info I need is never mentioned in the articles I read. Can anyone recommend some specific articles?
 * 1) What kind of a lipid modification does the wnt protein have and what other proteins share this modification?
 * 2) Are HDL particles loaded on the plasma membrane or in the cell?
 * 3) What is the function of the apolipoproteins (especially Apolipoprotein E)?
 * 4) What proteins are involved in the binding, endocytosis and secretion of HDL, and what diseases are related to deficiencies those proteins?
 * 5) How does lipoprotein transport and recycling work in mammals?

Remember, although answers to these questions are appreciated, articles that may help to answer these questions are welcomed even more. -- [[User:MacGyverMagic|Mgm|(talk)]] 07:09, Oct 25, 2004 (UTC)


 * Have you tried PubMed or similar databases? --inks 07:14, 25 Oct 2004 (UTC)


 * I have. But they return such a huge amount of articles that I really have no clue what to read first. Tips on which key words to search for are appreciated as well.


 * Search terms "lipid modification the wnt protein" returned a single article, the abstract appears to be relevant to part 1 of your question. Also found this one with "apoE function expression review". I find that a good way to do it is brute force your way through the PubMed results till you find a relevant paper, then go for the articles they reference. --inks 07:25, 25 Oct 2004 (UTC)


 * Thanks. I think I found the first one myself, I'll give the other one a read through. [[User:MacGyverMagic|Mgm|(talk)]] 07:55, Oct 25, 2004 (UTC)

For the more general answers on HDL metabolism I would recommend the latest edition of Scriver (Metabolic Basis of Inherited Disease) (2003 I think) which is available in all med school and many hospital libraries, often on the reference shelf. A medical librarian should immediately recognize the request as it is a multivolume "encyclopedic" reference work. This is a good place to start. If I have time later, I'll see what I can find in Medline on wnt. You can refine your search by restricting to "review" articles that mention wnt and lipoprotein. I'll bet that makes it a manageable number. If you are lucky, the text for one or more may be online.Alteripse 15:57, 25 Oct 2004 (UTC)

http://www.sigmaaldrich.com/Area_of_Interest/Biochemicals/Enzyme_Explorer/Key_Resources/Plasma__Blood_Protein/Apolipoprotein.html
 * Note: http://www.sigmaaldrich.com/Area_of_Interest/Biochemicals/Enzyme_Explorer/Key_Resources/Plasma__Blood_Protein/Lipoprotein_Function.html
 * Apolip function: