User:ManVhv/Sandbox/EBOV Proteins

The genome is non-segmented negative-sense RNA (nsnsRNA).

The gene order is 3′ – leader – NP – VP35 – VP40 – GP/sGP – VP30 – VP24 – L – trailer – 5′


 * NP
 * VP35
 * VP40
 * GP/sGP
 * VP30
 * VP24
 * L - "Large" polymerase - complex with VP35 constitutes RNA-dependent RNA polymerase (RdRp)
 * Determined Nucletoide sequence of L encoding gene and 5' end of RNA.
 * RNA has some folded structure, with self-complementary regions on 5' end, and 3' is complemetary to 5'.
 * L protein has conserved amino acid sequences with other nsnsRNA viruses
 * Conserved functionality is RNA-dependent RNA polymerase (RdRp)
 * VSV L protein is catalyst for 5'-end capping
 * VSV mRNA is capped with GDP instead of GTP
 * VSV L caps mRNA via "RNA:GDP polyribonucleotidyltransferase activity, in which a 5'-monophosphorylated viral mRNA-start sequence is transferred to GDP generated from GTP via a covalent enzyme-RNA intermediate"
 * VSV mRNA is capped with GDP instead of GTP
 * VSV L caps mRNA via "RNA:GDP polyribonucleotidyltransferase activity, in which a 5'-monophosphorylated viral mRNA-start sequence is transferred to GDP generated from GTP via a covalent enzyme-RNA intermediate"


 * L-L and L-VP35