User:Manninpk/cellular recording subpage

Cellular recording is a collection of methods devised to analyze the signaling patterns of cells.These techniques are found to be especially fundamental to the study of neurons. These methods measure the electrophysiological changes of neurons governed by action potential signaling, which are generated through variations in ion conduction. the introduction can also touch on the basis of the electrophysiology of neurons.

The history of cellular recording will also be analyzed as it took off in the mid 20th century: Rudimentary methods of measuring the electrophysiological properties of neurons were first practiced by Hodgkin and Katz with squid neuron in 1949. Advances in cellular recording devices allow for more precise measurements with the use of microelectrodes.

Intracellular recording refers to the measuring of voltage and current across a cell’s membrane. In this section we will describe how this recording is possible and explain various techniques used to measure the electrical properties of neurons. These techniques will include the voltage clamp, the current clamp, the patch-clamp, and sharp electrode methods. We will briefly describe each technique and express the strengths and weaknesses they provide.

Extracellular recording involves electrophysiological techniques that measure voltage and current changes outside of a cell from nerve fibers to brain slices. Various techniques including single-unit recording, field potentials, and aperometry will be discussed and analyzed. The methods, advantages, and disadvantages of each technique will also be discussed.

As a group, we plan to work together to complete this assignment, with each member playing an equal role. As of now, we have broken up our topic into three sections: (1) introduction, history, and conclusion; (2) intracellular recording; and (3) extracellular recording. Each member will be in charge of one section but will also aid teammates with their parts. We will constantly evaluate the research and writing workloads and will adjust roles based on these evaluations.

References: Purves, D. Neuroscience 4th Edition. Verkhratsky and Butt. Glial Neurobiology.