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Pasteurization or pasteurisation is a process in which packaged and non-packaged foods (such as milk and fruit juice) are treated with mild heat (<100°C) to eliminate pathogens and extend shelf-life. The process is intended to reduce spoilage organisms and eliminate vegetative bacteria but not bacterial spores.

This process was named after the French scientist Louis Pasteur, whose research in the 1880s demonstrated that thermal processing would inactivate unwanted microorganisms in wine. Spoilage enzymes are also inactivated during pasteurization.

Most liquid products are heat treated in a continuous system where heat can be applied using plate heat exchanger and/or direct or indirect use of steam and hot water. Due to the mild heat there are minor changes to the nutritional quality of foods as well as the sensory characteristics. The effects on quality and nutrition of pasteurized food products is related to the presence of heat labile vitamins in the foods. Other novel pasteurization techniques have been created, such as Pascalization or high pressure processing (HPP) and Pulsed Electric Field (PEF).

History
The process of heating wine for preservation purposes has been known in China since 1117, and was documented in Japan in the diary Tamonin-nikki, written by a series of monks between 1478 and 1618. Much later, in 1768, an Italian priest and scientist Lazzaro Spallanzani's research proved  a product could be "sterile" after thermal processing. He boiled meat broth for one hour, and the container was sealed immediately after boiling, and noticed that the broth did not spoil and was free from microorganisms.

In 1795, a Parisian chef and confectioner named Nicolas Appert began experimenting with ways to preserve foodstuffs, succeeding with soups, vegetables, juices, dairy products, jellies, jams, and syrups. He placed the food in glass jars, sealed them with cork and sealing wax and placed them in boiling water. In that same year, the French military offered a cash prize of 12,000 francs for a new method to preserve food. After some 14 or 15 years of experimenting, Appert submitted his invention and won the prize in January 1810. Later that year, Appert published "L'Art de conserver les substances animales et végétales" (or The Art of Preserving Animal and Vegetable Substances). This was the first cookbook of its kind on modern food preservation methods. La Maison Appert (The House of Appert), in the town of Massy, near Paris, became the first food-bottling factory in the world, preserving a variety of food in sealed bottles. Appert's method was to fill thick, large-mouthed glass bottles with produce of every description, ranging from beef and fowl to eggs, milk and prepared dishes. He left air space at the top of the bottle, and the cork would then be sealed firmly in the jar by using a vise. The bottle was then wrapped in canvas to protect it, while it was dunked into boiling water and then boiled for as much time as Appert deemed appropriate for cooking the contents thoroughly. Appert patented his method, sometimes called appertisation in his honor. Appert's method was so simple and workable that it quickly became widespread. In 1810, British inventor and merchant Peter Durand, also of French origin, patented his own method, but this time in a tin can, so creating the modern-day process of canning foods. In 1812, Englishmen Bryan Donkin and John Hall purchased both patents and began producing preserves. Just a decade later, Appert's method of canning had made its way to America. Tin can production was not common until the beginning of the 20th century, partly because a hammer and chisel were needed to open cans until the invention of a can opener by an inventor named Yates in 1855. A less aggressive method was developed by the French chemist Louis Pasteur during an 1864 summer holiday in Arbois. To remedy the frequent acidity of the local aged wines, he found out experimentally that it is sufficient to heat a young wine to only about 50 - 60 °C for a short time to kill the microbes, and that the wine could subsequently be aged without sacrificing the final quality. In honour of Pasteur, the process became known as "pasteurization". Pasteurization was originally used as a way of preventing wine and beer from souring, and it would be many years before milk was pasteurized. In the United States in the 1870s, it was common for milk to contain substances intended to mask spoilage before milk was regulated.

Milk
Milk is an excellent medium for microbial growth, and when stored at ambient temperature bacteria and other pathogens soon proliferate. The US Centers for Disease Control (CDC) says improperly handled raw milk is responsible for nearly three times more hospitalizations than any other food-borne disease source, making it one of the world's most dangerous food products. Diseases prevented by pasteurization can include tuberculosis, brucellosis, diphtheria, scarlet fever, and Q-fever; it also kills the harmful bacteria Salmonella, Listeria, Yersinia, Campylobacter, Staphylococcus aureus, and Escherichia coli O157:H7, among others.

Prior to industrialization, dairy cows were kept in urban areas to limit the time between milk production and consumption hence the risk of disease transmission via raw milk was reduced. As urban densities increased and supply chains lengthened to the distance from country to city, raw milk (often days old) became recognized as a source of disease. For example, between 1912 and 1937 some 65,000 people died of tuberculosis contracted from consuming milk in England and Wales alone. Because tuberculosis has a long incubation period in humans, it was difficult to link unpasteurized milk consumption as the cause to the effect of disease. In 1892, chemist Earnest Lederle experimentally inoculated milk from tuberculosis-diseased cows into Guinea pigs, which caused them to develop the disease. In 1910, Lederle, then in the role of Commissioner of Health, introduced mandatory pasteurization of milk in New York city.

Developed countries adopted milk pasteurization to prevent such disease and loss of life, and as a result milk is now widely considered one of the safest foods. A traditional form of pasteurization by scalding and straining of cream to increase the keeping qualities of butter was practiced in Great Britain before 1773 and was introduced to Boston in the British Colonies by 1773, although it was not widely practiced in the United States for the next 20 years. Pasteurization of milk was suggested by Franz von Soxhlet in 1886. In the early 20th century, Milton Joseph Rosenau established the standards (i.e. low temperature, slow heating at 60 °C (140 °F) for 20 minutes) for the pasteurization of milk  while at the United States Marine Hospital Service, notably in his publication of The Milk Question (1912). States in the U.S.A. began enacting mandatory dairy pasteurization laws with the first in 1947, and in 1973 the U.S. Federal Government required pasteurization of milk used in any interstate commerce.

The shelf life of refrigerated pasteurized milk is greater than that of raw milk. For example high-temperature, short-time (HTST) pasteurized milk typically has a refrigerated shelf life of two to three weeks, whereas ultra-pasteurized milk can last much longer, sometimes two to three months. When ultra-heat treatment (UHT) is combined with sterile handling and container technology (such as aseptic packaging), it can even be stored non-refrigerated for up to 9 months.

According to the United States Centers for Disease Control between 1998 and 2011, 79% of the dairy-related outbreaks were due to raw milk or cheese products. They report 148 outbreaks and 2,384 illnesses (284 requiring hospitalizations), as well as two deaths due to raw milk or cheese products during the same time period.

Pasteurization process
Pasteurization is a mild heat treatment of liquid foods (both packaged and unpackaged) where products are typically heated to below 100°C. The heat treatment and cooling process are designed to inhibit a phase change of the product. The acidity of the food determines the parameters (time and temperature) of the heat treatment as well as the duration of shelf-life. Parameters also take into account nutritional and sensory qualities that are sensitive to heat.

In acidic foods (pH<4.6), such as fruit juice and beer, the heat treatments are designed to inactivate enzymes (pectin methylesterase and polygalacturonase in fruit juices) and destroy spoilage microbes (yeast, lactobacillus). Due to the low pH of acidic foods, pathogens are unable to grow. The shelf-life is extended several weeks. In low-acid foods (pH>4.6), such as milk and liquid eggs, the heat treatments are designed to destroy pathogens and spoilage organisms (yeast and molds). It is important to note that not all spoilage organisms are destroyed under pasteurization parameters thus requiring refrigeration.

Equipment
Food can be pasteurized in two ways: either after being packaged into containers or before being packaged into containers.

When food is packaged in glass, hot water is used to lower risk of thermal shock. Plastics and metals are also used to package foods, and these are packaged using steam or hot water since the risk of thermal shock is low.

Most liquid foods are pasteurized using continuous systems that have a heating zone, hold tube, and cooling zone, after which the product is filled into the package. Plate heat exchangers are used for low viscosity products such as milk, nut milks and juices. A plate heat exchanger is composed of many thin vertical stainless steel plates, which separate the liquid from the heating or cooling medium. Scraped surface heat exchangers contain an inner rotating shaft in the tube, and serves to scrape high viscous material which might accumulate on the wall of the tube. Shell or tube heat exchanger s are designed for the pasteurization of Non-Newtonian foods such as dairy products, tomato ketchup and baby foods. A tube heat exchanger is made up of concentric stainless steel tubes. Food passes through the inner tube while the heating/cooling medium is circulated through the outer or inner tube.

The benefits of a using heat exchanger to pasteurize non packaged foods versus pasteurizing foods in containers are:


 * Heat exchanger s provide uniform treatment;There is greater flexibility with regards to the products which can be pasteurized on these plates
 * The process is more energy efficient compared to pasteurizing foods in packaged containers.
 * Greater throughput

After being heated in a heat exchanger, the product flows through a hold tube for a set period of time to achieve the required treatment. If pasteurization temperature or time is not achieved, a flow diversion valve is utilized to divert under-processed product back to the raw product tank. If the product is adequately processed, it is cooled in a heat exchanger, then filled.

High temperature short time (HTST) pasteurization, such as that used for milk (71.5°C for 15 seconds) ensures safety of milk and provides a refrigerated shelf life of approximately two weeks. In UHT pasteurization, milk is pasteurized at 135°C for 1-2 seconds which provides the same level of safety, but along with the packaging, extends shelf life to 3 months under refrigeration.

Verification
Direct microbiological techniques are the ultimate measurement of pathogen contamination, but these are costly and time-consuming, which means that products have a reduced shelf-life by the time pasteurization is verified.

As a result of the unsuitability of microbiological techniques, milk pasteurization efficacy is typically monitored by checking for the presence of alkaline phosphatase, which is denatured by pasteurization. Destruction of alkaline phosphatase ensures the destruction of common milk pathogens. Therefore, the presence of alkaline phosphatase is an ideal indicator of pasteurization efficacy. For liquid eggs, the effectiveness of the heat treatment is measured by the residual activity of α-amylase.

Efficacy against pathogenic bacteria
During the early 20th century there was no robust knowledge of what time and temperatures combinations would inactivate pathogenic bacteria in milk, and so a number of different pasteurization standards were in use. By 1943, both HTST pasteurization conditions of 72 °C for 15 seconds, as well as batch pasteurization conditions of 63 °C for 30 minutes, were confirmed by studies of the complete thermal death (as best as could be measured at that time) for a range of pathogenic bacteria in milk. Complete inactivation of Coxiella burnetii (which was thought at the time to cause Q fever by oral ingestion of infected milk) as well as of Mycobacterium tuberculosis (which causes tuberculosis) were later demonstrated. For all practical purposes, these conditions were adequate for destroying almost all yeasts, molds, and common spoilage bacteria and also to ensure adequate destruction of common pathogenic, heat-resistant organisms. However, the microbiological techniques used until the 1960s did not allow for the actual reduction of bacteria to be enumerated. Demonstration of the extent of inactivation of pathogenic bacteria by milk pasteurization came from a study of surviving bacteria in milk that was heat treated after being deliberately spiked with high levels of the most heat-resistant strains of the most significant milk-borne pathogens.

The mean log10 reductions and temperatures of inactivation of the major milk-borne pathogens during a 15-s treatment are:


 * Staphylococcus aureus >6.7 at 66.5 °C
 * Yersinia enterocolitica >6.8 at 62.5 °C,
 * pathogenic Escherichia coli >6.8 at 65 °C
 * Cronobacter sakazakii >6.7 at 67.5 °C
 * Listeria monocytogenes >6.9 at 65.5 °C, and
 * Salmonella ser. Typhimurium >6.9 at 61.5 °C.

The Codex Alimentarius Code of Hygienic Practice for Milk notes that milk pasteurization is designed to achieve at least a 5 log10 reduction of Coxiella burnetii. The Code also notes that: “The minimum pasteurization conditions are those having bactericidal effects equivalent to heating every particle of the milk to 72°C for 15 seconds (continuous flow pasteurization) or 63°C for 30 minutes (batch pasteurization)” and that “To ensure that each particle is sufficiently heated, the milk flow in heat exchangers should be turbulent, i.e. the Reynolds number should be sufficiently high.” The point about turbulent flow is important because simplistic laboratory studies of heat inactivation that use test tubes, without flow, will have less bacterial inactivation than larger scale experiments that seek to replicate conditions of commercial pasteurization. As a precaution, modern HTST pasteurization processes must be designed with flow-rate restriction as well as divert valves which ensure that the milk is heated evenly, and no part of the milk is subject to a shorter time or a lower temperature. It is common for the temperatures to exceed 72 °C by 1.5 °C or 2 °C.

Effects on nutritional characteristics of foods
Pasteurization, because of its mild heat treatment, increases the shelf-life by a few days or weeks. However, this mild heat also means there are only in minor changes to the nutritional quality of foods. The effects on quality and nutrition of pasteurized food products is related to the presence of heat labile vitamins in the foods.

Milk
According to a systematic review and meta-analysis, it was found that pasteurization appeared to qualitatively reduce concentrations of vitamins B12 and E, but it did increase concentrations of vitamin A. Apart from meta-analysis, it's not possible to conclude the effect of pasteurization on vitamins A, B12, and E based merely on consultation of the vast literature available.

Milk is not an important source of vitamins B12 or E in the North American diet, so the effects of pasteurization on the adult daily intake of these vitamins is negligible. However, milk is considered an important source of vitamin A, and because pasteurization appears to increase vitamin A concentrations in milk, the effect of milk heat treatment on this vitamin is a not a major public health concern. Results of meta-analyses revealed that pasteurization of milk leads to a significant decrease in vitamin C and folate, but milk also is not an important source of these vitamins. However, a significant decrease in vitamin B2 concentrations was found after pasteurization. Vitamin B2 is typically found in bovine milk at concentrations of 1.83 mg/liter. Because the recommended daily intake for adults is 1.1 mg/day, milk consumption greatly contributes to the recommended daily intake of this vitamin. With the exception of B2, pasteurization does not appear to be a concern in diminishing the nutritive value of milk because milk is often not a primary source of these studied vitamins in the North American diet.

Juices
Pasteurization may result in quality reduction in the case of fruit juices, as a result of the loss of volatile aroma compounds during the process. However, fruit juice products undergo a deaeration process prior to pasteurization that may be responsible for this loss. Deaeration also minimizes the loss of nutrients like vitamin C and carotene. To prevent the quality loss resulting from the loss in volatile compounds, volatile recovery, though costly, can be used utilized to produce higher quality juice products.

Sensory effects on food
Pasteurization, because it is a mild heat treatment, also has little effect on the sensory attributes of the foods that are processed. In regards to color, the pasteurization process doesn't have much effect on pigments such as chlorophyll, anthocyanin and carotenoid in plants and animal tissues. In fruit juices, polyphenol oxidase (PPO) is the main enzyme responsible for causing browning and color changes. However, this enzyme is deactivated in the deaeration step prior to pasteurization with the removal oxygen.

In milk, the color difference between pasteurized and raw milk is related to the homogenization step that takes prior to pasteurization. Before pasteurization, milk is homogenized to separate the solids (fat) from the liquid, which results in the pasteurized milk having a whiter appearance compared to raw milk.

For vegetable products, color degradation is dependent on the temperature conditions and the duration of heating. In vegetable products, pasteurization may result in some textural loss as a result of enzymatic and non enzymatic transformations in the structure of pectin if the processing temperatures are too high as a result. However, with mild heat treatment pasteurization, tissue softening in the vegetables that cause textural loss is not of concern as long as the temperature does not get above 80°C.

Novel pasteurization
Other thermal and non thermal processes have been developed to pasteurize foods as a way of reducing the affects on nutritional and sensory characteristics of foods and preventing degradation of heat labile nutrients. Pascalization or high pressure processing (HPP) and Pulsed Electric Field (PEF) are examples of these new pasteurization methods that are currently commercially utilized.

Microwave volumetric heating (MVH) is the newest available pasteurization technology. It uses microwaves to heat liquids, suspensions, or semi-solids in a continuous flow. Because MVH delivers energy evenly and deeply into the whole body of a flowing product, it allows for gentler and shorter heating, so that almost all heat-sensitive substances in the milk are preserved.

Products that are commonly pasteurized

 * Beer
 * Canned food
 * Dairy products
 * Eggs
 * Milk
 * Juices
 * Low alcoholic beverages
 * Syrups
 * Vinegar
 * Water
 * Wines
 * Nuts