User:Mdm2203/sandbox

Isolation of OSCs
Putative mammalian oogonial stem cells have been isolated from ovarian cortex tissue following two-step enzymatic digestion, then separated from other ovarian cells using immunofluorescent (FACS) and immunomagnetic (MACS) cell sorting techniques.

Culture of OSCs
Culturing OSCs in vitro requires replication of in situ conditions for successful development. OSCs were cultured onto a medium containing multiple growth factors and nutrients which are regularly changed and subcultured to avoid cell death.

OSCs have been labelled with GFP to allow them to be traced when transplanted into animals in subsequent experiments.