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Next Generation Sequencing Methods
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RNA Modification Function
Transfer RNA is the most abundantly modified type of RNA. Modifications in tRNA play crucial roles in maintaining translation efficiency through supporting structure, anticodon-codon interactions, and interactions with enzymes.

Anticodon modifications are important for proper decoding of mRNA. Since the genetic code is degenerate, anticodon modifications are necessary to properly decode mRNA. Particularly, the wobble position of the anticodon determines how the codons are read. For example, in eukaryotes when there is an adenosine (A) at position 34 (wobble) of the anticodon, A can be converted to inosine (I). Inosine is a modification that is able to base-pair with cytosine, adenine, and uridine.

Another commonly modified base in tRNA in the position adjacent to the anticodon. Position 37 is often hypermodified with bulky chemical modifications. These modifications prevent frameshifting and increase anticodon-codon binding stability through stacking interactions.

RNA Modification Naming
Nomenclature for the naming of RNA modifications takes into account two factors:


 * 1) The location on the base of the chemical modifications
 * 2) The type of modification

RNA Modification Types
Modifications can range from simple methylations (m6A) to hypermodifications (i6A) that require several steps for synthesis.