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The poet Theodore Roethke wrote about plant cuttings and root growth behavior in his poems "Cuttings" and "Cuttings (Later)" found in his book The Lost Son: And Other Poems.

= Evolutionary advantage: succulents = Cuttings are used as a method of asexual reproduction in succulent horticulture, commonly referred to as vegetative reproduction. A cutting can also be referred to as a progagule. Succulents have evolved with the ability to use adventitious root formation in reproduction to increase fitness in stressful environments. Succulents grow in shallow soils, rocky soils, and desert soils. Seedlings from sexual reproduction have a low survival rate; however, plantlets from the excised stem cuttings and leaf cuttings, broken off in the natural environment, are more successful. Cuttings have both water and carbon stored and available, which are resources needed for plant establishment. The detached part of the plant remains physiologically active, allowing mitotic activity and new root structures to form for water and nutrient uptake. Asexual reproduction of plants is also evolutionarily advantageous as it allows plantlets to be better suited to their environment though retention of epigenetic memory, heritable patterns of phenotypic differences that are not due to changes in DNA but rather histone modification and DNA methylation. Epigenetic memory is heritable through mitosis, and thus advantageous stress response priming is retained in plantlets from excised stem.

= Physiology = Adventitious root formation refers to roots that form from any structure of a plant that is not a root; these roots can form as part of normal development or due to a stress response. Adventitious root formation from the excised stem cutting is a wound response.

At a molecular level when a cutting is first excised at the stem there is an immediate increase in jasmonic acid, known to be necessary for adventitious root formation. When the cutting is excised from the original root system the root inhibiting hormones, cytokinin and strigolactone, which are made in the root and transported to the stem, decrease in concentration. Polyphenol degradation decreases, increasing auxin concentration. The increased auxin concentration increases nitric oxide concentration which initiates root formation through a MAPK signal cascade and a cGMP-dependent pathway that that both regulate mitotic division and are both necessary for the initiation of adventitious root formation. The root primordia form from cambial cells in the stem. In propagation of detached succulent leaves and leaf cuttings, the root primordia typically emerges from the basal callous tissue after the leaf primordia emerges.

It was known as early as 1935 that when indolyl-3-acetic acid (IAA), also known as auxin, is applied to the stem of root cuttings, there is an increase the average number of adventitious roots compared to cuttings that are not treated. Researchers also applied this compound to stems without leaves that normally would not have any root formation and found that auxin induced root formation, thus determining auxin is necessary for root formation. Identification of this hormone has been important to industries that rely on vegetative propagation, as it is sometimes applied to fresh cuttings to stimulate root growth.