User:Mistyyrain/sandbox

1.) Gene
SH2 domain containing protein 4B is a protein which in humans is encoded by the SH2D4B gene. It maps on chromosome 10, at 10q23.1 and covers 108.66kb from 82297657. It SH2D4B gene is located at the carboxyl ends of proteins and show the highest degree of homology to SH2D4A. The amino-terminal region of SH2D4B contains three conserved tyrosine residues I n the consensus of phosphorylation motifs and a proline-rich stretch located in the same position as that found in SH2D4A. It could be that SH2D4B substitutes for the function of SH2D4A in cells deficient of this T-cell. The paper emphasizes that there is no information on protein expression of data nor the role of SH2D4B in cells.

2.) mRNA
The official symbol of this protein is SH2D4B. The accession number of the protein is NM_207372, Version NM_207372.2. The mRNA is 3983 base pairs long. The protein is 357 amino acids long. The protein’s isoform 1 has a distinct first exon that results in a distinct N-terminus. This is on account of the inclusion of a translation initiation site that is included, which is inferred.

3.) Protein

The SH2 domain contains about 100 amino-acid residues. It functions as regulatory modules of intracellular signaling cascades through interacting with high affinity to phosphotyrosine-containing target peptides in a specific sequence. The domain is shaped in a compact flattened hemisphere, consisting of a central anti-parallel hydrophobic beta sheet that is surrounded by two short alpha helices.

4.) Clinical Significance

In 2018, an RNA sequence analysis was performed on tissue samples from ninety-five individuals that represented twenty seven different tissues in order to determine the tissue specificity of all the different protein coding genes. Part of this included RNA sequencing of the total RNA from twenty human tissue samples, and the highest RPKM of the samples was brain fetal samples with a mean RPKM of 0.166.

The illumina bodyMap2 transcriptome was evaluated by transcription profiling in high throughput sequencing of individuals and a mixture of the RNA of sixteen human tissues. The highest mean RPKM was in the testes with 0.294. The end of this analysis concluded that through evaluation of the protein coding, the SH2 domain containing 4B is predicted to be active in the cytoplasm of the gene.

The Allen Brain Atlas developing human brain tissue gene expression profiles samples the mRNA expression profiles for the human brain tissue samples in twenty-seven different points of time across twenty-six different brain structures. The gene expression by microarray was measured.