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= C8orf46 = Chromosome 8 open reading frame 46, C8orf46, is an un-characterized protein coding gene located along the forward strand of chromosome 8.

Location
C8orf46 is found along the plus strand of chromosome 8 (8q13.1) with the genomic sequence beginning at 66,460,003 bp and ending at 66,518,524. The entire gene is 58,522 bp long. C8orf46 is flanked by alcohol dehydrogenase, iron containing 1 and v-myb avian myeloblastosis viral oncogene homolog-like.

Expression
C8orf46 is ubiquitously expressed in the body. The gene is expressed in 13 different types of tissue throughout the body, with the brain, spinal cord and nerves showing elevated expression of the gene. Specifically, the isocortex and hippocampal formation areas of the brain showed high levels of expression. In addition to healthy tissue, C8orf46 is also found in several disease state tumors, including chondrosarcoma, glioma, kidney, liver, and germ cells. C8orf46 is only expressed in infants and adults.

Homology
C8orf46 is found in all classes of vertebrates, including mammals, birds, fish, reptiles and amphibians. However, orthologs are only present in a select few fish and amphibians. The gene has not been found in any plants, fungi, and single celled organisms. No human paralogs for C8orf46 have been identified.

Splice Variants
C8orf46 has only one mRNA splice variant, and is approximately 3741 base pairs in length. The mature mRNA contains 6 exons, and has the accession number NM_152765.3.

General Properties
The protein encoded by C8orf46 is 207 amino acids long, which equates to a molecular weight of 22.6 kdal. The isoelectric point of the protein is 10.42099 which indicates the pH level at which the protein is neutral. The protein of C8orf46 does contain a domain of unknown function (DUF4648), and is a part of the SH3 domain family, which is known to bind to proline-rich ligands.

Composition
The protein derived from C8orf46 is considered rich in arginine, and poor in phenylalanine compared to the composition of the average human protein. C8orf46 does contain several regions of positively charged runs, and has a high concentration of basic amino acids.

Post Translation Modification
C8orf46 is predicted to undergo several types of post translational modifications. With a high degree of certainty, it is predicted that C8orf46 undergoes O-glycosylation, serine, threonine and tyrosine phosphorylation, sumolyation and initial methionine acetylation.

Subcellular Location
C8orf46 does not contain any transmembrane domains suggesting that it is an intracellular protein. C8orf46 is predicted to be a nuclear protein, given the lack of signal peptides and transmembrane domains.