User:Mmoquia/sandbox

HeLa cells were used to investigate the phytochemical compounds and the fundamental mechanism of the anticancer activity of the ethanolic extract of mango peel (EEMP). EEMP was found to contain various phenolic compounds and to activate death through apoptosis of human cervical malignant HeLa cells which suggests EEMP may help to prevent cervical cancer as well as other types of cancers.

Knowing cancer prevents the occurrence of apoptosis, scientists study natural products and strive to obtain their apoptosis-activating promoters for usage in anticancer medication. Mango, or Mangifera indica, is a tropical and subtropical grown fruit popular for its vibrant color, unique taste, and nutrient content. Mango peel is 15-20% of the overall mass of mango fruit. Different classes of polyphenols, carotenoids, and vitamins are found in mango fruit. These compounds contain various health-promoting properties such as antioxidant activity. The mango peel consists of more polyphenols than the mango flesh.

Ethanolic extract (fragrance extraction) of mango peel was found to contain high antioxidant.

Ethanolic extract of mango peel (EEMP) is found to be a good source of quercetin 3-O-galactoside, mangiferin gallate, isomangiferin gallate, quercetin-3-O-arabinopyranoside, and mangiferin as well as unsaturated fatty acids oleic acid, linoleic acid, and ethyl linoleate according to studies using liquid chromatography-mass spectrometry. The activated death through apoptosis of human cervical malignant HeLa cells is evident by high growth of cells in the sub-G1 phase and the display of fragmented nuclei. EEMP also decreases anti-apoptic Bcl-2 expression which influences proteolytic activation of caspase-3, 7, 8, and 9 along with the reduction of poly ADP-ribose polymerase protein. Studies also claim of EEMP that both extrinsic and intrinsic pathways are involved in the apoptosis activation of HeLa cells.