User:Mruss22/chthonomonas calidirosea sandbox

Chthonomonas calidirosea is a Gram-negative bacterium and also the first representative of the new class Chthonomonadetes within the phylum Armatimonadetes. The Armatimonadetes were previously known as candidate phylum OP10. OP10 was comprised solely of environmental 16S rRNA gene clone sequences prior to C. calidirosea's relative, Armatimonas rosea's discovery.

Discovery
C. calidirosea was originally isolated from geothermally heate soil at Hell's Gate, Tikitere, New Zealand. The soil was aseptically sampled at an approximate depth of 15 cm below the surface. The sampled soil had a pH of 4.3 and an in situ temperature of 55oC.

Relatives
The environmental 16S rRNA gene sequences, belonging to the phylum Armatimonadetes are currently sorted into six groups. Groups 2, 5, and 6 consist solely of sequences. Group 1 contains Armatimonadetes rosea, Group 3 contains Chthonomonadetes calidirosea, and Group 4 conatins Fimbriimonas ginsengisoli.

Armatimonas rosea an aerobic, Gram-negative, pink pigmented, nonmotile,ovoid/rod shaped bacterium, was isolated from the rhizoplane of an aquatic plan Phragmites australis in Japan. It is the first representative of the phylumArmatimonadetes.

Fimbriimonas ginsengisoli, an aerobic, non-motile, mesophilic, rod-shaped bacterium, was isolated from a ginseng field soil sample. It represents the first cultured representative of the Fimbriimonadia class, corresponding with Group 4 of the phylumArmatimonadetes.

Characteristics
When grown on solid medium AOM1, colonies were light-pink pigmented, circular, convex, relatively small (2-4 mm) in size after one week of incubation at 60oC. A.rosea has an optimum temperature at 68oC with a range of 50-73oC. The optimum pH for growth was 5.3 with a range of 4.5-5.8. Optimum growth was observed in NaCl free medium, although growth was observed in NaCl amended FSV1 medium up to 2% NaCl.

When examined by phase-contrast and transmission electron microscopy a limited number of cells were observed to exhibit a tumbling motility that was highly dependent on growth phase, medium pH and substrate. An irregular, corrugated outer membrane was consistently observed. Cells were rod shaped, with sizes ranging from 0.5-0.7 µm in diameter and 2.5-3.0 µm in length. Neither spores nor flagella were observed.

Cultures of C. calidirosea were observed to be aerobic, saccharolytic, obligately thermophilic, motile, non-spore-forming bacterium with no evidence of growth under anaerobic conditions.